The expression pattern of PFKFB3 enzyme distinguishes between induced-pluripotent stem cells and cancer stem cells
| Autor: | Joanna Rzeszowska-Wolny, Mayur V. Jain, Artur Cieślar-Pobuda, Gunnar Kratz, Saeid Ghavami, Emilia Wiechec |
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| Rok vydání: | 2015 |
| Předmět: |
cancer stem cells
PFKFB3 R-point induced pluripotent stem cells Cell type Phosphofructokinase-2 Phosphofructokinase-1 Cell- och molekylärbiologi Immunoblotting Cell Biology Stem cell marker Cancer stem cell Cell Line Tumor Neoplasms Spheroids Cellular medicine Humans Induced pluripotent stem cell Cells Cultured Microscopy Confocal Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Cell Cycle Aldehyde Dehydrogenase Cell cycle Cell biology PFK1 medicine.anatomical_structure Oncology Cancer cell Neoplastic Stem Cells RNA Interference Stem cell Cell and Molecular Biology Research Paper |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| DOI: | 10.18632/oncotarget.4995 |
| Popis: | Induced pluripotent stem cells (iPS) have become crucial in medicine and biology. Several studies indicate their phenotypic similarities with cancer stem cells (CSCs) and a propensity to form tumors. Thus it is desirable to identify a trait which differentiates iPS populations and CSCs. Searching for such a feature, in this work we compare the restriction (R) point-governed regulation of cell cycle progression in different cell types (iPS, cancer, CSC and normal cells) based on the expression profile of 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase3 (PFKFB3) and phosphofructokinase (PFK1). Our study reveals that PFKFB3 and PFK1 expression allows discrimination between iPS and CSCs. Moreover, cancer and iPS cells, when cultured under hypoxic conditions, alter their expression level of PFKFB3 and PFK1 to resemble those in CSCs. We also observed cell type-related differences in response to inhibition of PFKFB3. This possibility to distinguish CSC from iPS cells or non-stem cancer cells by PFKB3 and PFK1 expression improves the outlook for clinical application of stem cell-based therapies and for more precise detection of CSCs. |
| Databáze: | OpenAIRE |
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