Photodetection of DNA mismatches by dissymmetric Ru(ii) acridine based complexes
| Autor: | Jérôme Dejeu, Garry S. Hanan, Frédérique Loiseau, Lionel Marcelis, Quentin Deraedt, Eric Defrancq, Baptiste Laramée-Milette, Benjamin Elias, Martin Gillard |
|---|---|
| Přispěvatelé: | UCL - SST/IMCN/MOST - Molecules, Solids and Reactivity, Institut de la matière condensée et des nanosciences / Institute of Condensed Matter and Nanosciences (IMCN), Université Catholique de Louvain = Catholic University of Louvain (UCL), Département de chimie [UdeM-Montréal], Université de Montréal (UdeM), Département de Chimie Moléculaire - Chimie Inorganique Redox Biomimétique (DCM - CIRE ), Département de Chimie Moléculaire (DCM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Département de Chimie Moléculaire - Ingéniérie et Intéractions BioMoléculaires (DCM - I2BM) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Chemistry
chemistry.chemical_element Context (language use) 02 engineering and technology [CHIM.INOR]Chemical Sciences/Inorganic chemistry 010402 general chemistry 021001 nanoscience & nanotechnology Ligand (biochemistry) 01 natural sciences Combinatorial chemistry 0104 chemical sciences Ruthenium Inorganic Chemistry [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry chemistry.chemical_compound Excited state Acridine DNA mismatch repair [CHIM.COOR]Chemical Sciences/Coordination chemistry 0210 nano-technology Luminescence DNA |
| Zdroj: | Inorganic Chemistry Frontiers, Vol. 6, no. 9, p. 2260-2270 (2019) Inorganic Chemistry Frontiers Inorganic Chemistry Frontiers, Royal Society of Chemistry, 2019, 6 (9), pp.2260-2270. ⟨10.1039/c9qi00133f⟩ |
| ISSN: | 2052-1553 |
| Popis: | International audience; The early detection of DNA mutations such as DNA mismatches is of major interest. Indeed, the accumulation of mismatches into the genome arises from deficiencies of the cellular mismatch repair machinery that is often associated with several types of cancers being resistant to classic chemotherapeutics. In this context, ruthenium(II) compounds bearing a planar extended ligand appear to be excellent candidates as DNA photoprobes since they exhibit high affinity for DNA as well as tuneable luminescence properties. Herein, we report on the synthesis of a novel dissymmetric acridine based Ru(II) complex, [Ru (bpy) 2 napp] 2+ , along with the study of its ability to photodetect DNA mismatches. We also investigated the origin of the ability of the complex to photodetect mismatches via CD-melting assays and bio-layer interferometry. Interestingly, this behaviour may be attributed to a better protection of the excited state of the complex from non-radiative deexcitation sources (e.g., collisions with the solvent, oxygen photosensi-tization, etc.) when intercalated into well-matched compared to mismatched DNA. |
| Databáze: | OpenAIRE |
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