COVID-19: molecular and serological detection methods
| Autor: | Muna A. Abdal Rhida, Ahmed E. Dhamad |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Emergency Use Authorization
medicine.medical_specialty Coronavirus disease 2019 (COVID-19) Epidemiology Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) rRT-PCR lcsh:Medicine medicine.disease_cause Microbiology General Biochemistry Genetics and Molecular Biology World health Serology Isothermal amplification 03 medical and health sciences 0302 clinical medicine Virology CRISPR-Cas12 Pandemic medicine Pathology 030212 general & internal medicine Intensive care medicine 030304 developmental biology Coronavirus 0303 health sciences business.industry SARS-CoV-2 General Neuroscience lcsh:R COVID-19 General Medicine Gold standard (test) Infectious Diseases ID NOW COVID-19 ELISA LFA General Agricultural and Biological Sciences business |
| Zdroj: | PeerJ, Vol 8, p e10180 (2020) PeerJ |
| ISSN: | 2167-8359 |
| Popis: | Since COVID-19, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), was declared as a pandemic disease by the World Health Organization in early 2020, many countries, organizations and companies have tried to find the best way to diagnose the virus and contain its spreading. SARS-CoV-2 is a positive-sense single RNA (+ssRNA) coronavirus and mainly spreads through droplets, respiratory secretions, and direct contact. The early detection of the virus plays a central role in lowering COVID19 incidents and mortality rates. Thus, finding a simple, accurate, cheap and quick detection approach for SARS-CoV-2 at early stage of the viral infection is urgent and at high demand all around the world. The Food and Drug Administration and other health agencies have declared Emergency Use Authorization to develop diagnostic methods for COVID-19 and fulfill the demand. However, not all developed methods are appropriate and selecting a suitable method is challenging. Among all detection methods, rRT-PCR is the gold standard method. Unlike molecular methods, serological methods lack the ability of early detection with low accuracy. In this review, we summarized the current knowledge about COVID-19 detection methods aiming to highlight the advantages and disadvantages of molecular and serological methods. |
| Databáze: | OpenAIRE |
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