Posttranscriptional control of Klebsiella pneumoniae nif mRNA stability by the nifL product
| Autor: | W J Brill, G P Roberts, J J Collins |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Transcription
Genetic MRNA destabilization Acetates environment and public health Microbiology chemistry.chemical_compound Bacterial Proteins Nitrogen Fixation RNA Messenger Molecular Biology Derepression Regulation of gene expression Messenger RNA Models Genetic biology Temperature Wild type biochemical phenomena metabolism and nutrition biology.organism_classification Enterobacteriaceae Molecular biology Post-transcriptional modification Oxygen Klebsiella pneumoniae RNA Bacterial Gene Expression Regulation chemistry Genes Bacterial bacteria DNA Research Article |
| Zdroj: | Journal of Bacteriology. 168:173-178 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.168.1.173-178.1986 |
| Popis: | Posttranscriptional control of nif mRNA stability was demonstrated by functional and chemical analyses, using specific probes for four nif transcripts. In the wild type, nif transcripts (except nifLA) were stable during derepression, with half-lives of approximately 30 min. They were dramatically destabilized by O2 or elevated temperature (41 degrees C) and to a lesser extent by NH4+. In contrast, the nifLA message was not particularly stable, and posttranscriptional control was not evident. In NifL- strains, both forms of analysis indicated that the nifL product was involved in nif mRNA destabilization in the presence of O2 and NH4+. |
| Databáze: | OpenAIRE |
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