Pramlintide Antagonizes Beta Amyloid (Aβ)- and Human Amylin-Induced Depression of Hippocampal Long-Term Potentiation
| Autor: | Ryoichi Kimura, Jack H. Jhamandas, Jaynie F. Yang, David MacTavish, David Westaway |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine endocrine system medicine.medical_specialty Mice 129 Strain Amyloid Long-Term Potentiation Neuroscience (miscellaneous) Amylin Mice Transgenic Hippocampal formation Hippocampus Mice 03 medical and health sciences Cellular and Molecular Neuroscience Organ Culture Techniques 0302 clinical medicine Internal medicine Amyloid precursor protein medicine Animals Humans Amino Acid Sequence Amyloid beta-Peptides biology Chemistry musculoskeletal neural and ocular physiology Long-term potentiation Pramlintide Peptide Fragments Islet Amyloid Polypeptide 030104 developmental biology Endocrinology nervous system Neurology Synaptic plasticity biology.protein Excitatory postsynaptic potential Female 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Molecular Neurobiology. 54:748-754 |
| ISSN: | 1559-1182 0893-7648 |
| Popis: | Accumulation of amyloid-β peptide (Aβ) is a pathological hallmark of Alzheimer’s disease (AD). We have previously demonstrated that electrophysiological and neurotoxic effects of Aβ and human amylin are expressed via the amylin receptor. Recently, pramlintide, a synthetic analog of amylin, has been reported to improve cognitive function in transgenic AD mouse models. In this study, we examined the effects of pramlintide on Aβ1–42 and human amylin-evoked depression of long-term potentiation (LTP) at Schaeffer collateral-CA1 hippocampal synapses. In mouse hippocampal brain slices, field excitatory postsynaptic potentials (fEPSPs) were recorded from the stratum radiatum layer of the CA1 area in response to electrical stimulation of Schaeffer collateral afferents and LTP induced by 3-theta-burst stimulation (TBS) protocol. Aβ1–42 (50 nM) and human amylin (50 nM), but not Aβ42–1 (50 nM), depressed LTP. Pre-application of pramlintide (250 nM) blocked Aβ- and human amylin-induced reduction of LTP without affecting baseline transmission or LTP. We also examined the effects of pramlintide on LTP in transgenic mice (TgCRND8) that over-express amyloid precursor protein. In contrast to wild-type controls, where robust LTP was observed, 10- to 12-month-old TgCRND8 mice show blunted LTP. In TgCRND8 mice, basal LTP is enhanced by application of pramlintide. Our data indicate that pramlintide acts as a functional amylin receptor antagonist to reverse the effects of Aβ1–42 and human amylin on LTP and also increases LTP in transgenic mice that demonstrate increased ambient brain amyloid levels. Amylin receptor antagonists may thus serve as potentially useful therapeutic agents in treatment of AD. |
| Databáze: | OpenAIRE |
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