Simultaneous multiple immunoassays in a compact disc-shaped microfluidic device based on centrifugal force
| Autor: | Per Andersson, Nobuo Honda, Hiroyuki Takei, Ulrika Lindberg, Stephan Hoffmann |
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| Rok vydání: | 2005 |
| Předmět: |
Clinical Biochemistry
Serum albumin Analytical chemistry Centrifugation Sensitivity and Specificity Antibodies Carcinoembryonic antigen medicine Animals Bovine serum albumin Detection limit Immunoassay Chromatography biology medicine.diagnostic_test Chemistry Interleukin-6 Biochemistry (medical) Serum Albumin Bovine Microfluidic Analytical Techniques Fluorescence Carcinoembryonic Antigen Biotinylation biology.protein Cattle alpha-Fetoproteins |
| Zdroj: | Clinical chemistry. 51(10) |
| ISSN: | 0009-9147 |
| Popis: | Background: We explored the potential of a microfluidic device based on centrifugal force as an immunoassay platform by examining the imprecision of assays carried out with 200 nL of sample.Methods: Biotinylated antibodies against α-fetoprotein (AFP), interleukin-6 (IL-6), and carcinoembryonic antigen [(CEA); 0.1 g/L each in 15 mmol/L phosphate-buffered saline (PBS) containing 0.1 mL/L Tween 20] were attached to a microcolumn packed with streptavidin-coated particles. A 200-nL sample was then allowed to pass through the microcolumn for 240 s, followed by Alexa 647–labeled detection antibody (7.5 mg/L in 15 mmol/L PBS containing 10 g/L bovine serum albumin). The flow rate was controlled by altering the rotational speed. Up to 104 sandwich type immunoassays were completed within 50 min.Results: For AFP, IL-6, and CEA the detection limits were, respectively, 0.15, 1.25, and 1.31 pmol/L. Inter- and intraassay imprecisions (CVs) were 5 pmol/L. The CEA antibody had the lowest affinity according to fluorescence image analysis of the microcolumn region. The result was confirmed in a comparative study using BIAcore 3000.Conclusions: Day-to-day (total) imprecision (CV) of immunoassays on the compact disc–shaped device are |
| Databáze: | OpenAIRE |
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