Bovine brain factor promotes neurite extension from chick embryo dorsal root ganglia in vitro

Autor:	O. Sotnikov, N. Chalisova, V. Goncharova, G. Akoev, A. Romanjuk, E. Chumasov
Rok vydání:	1987
Předmět:	Brain Chemistry Dorsum Neurite General Neuroscience Embryo Chick Embryo Dendrites Biology Molecular biology In vitro Molecular Weight Tissue culture Bovine brain Affinity chromatography Ganglia Spinal Animals Bioassay Cattle Nerve Growth Factors Cells Cultured Subcellular Fractions
Zdroj:	Neuroscience Letters. 82:1-5
ISSN:	0304-3940
Popis:	A protein with neurite-stimulating activity was isolated from bovine brain tissue lysosomal fraction. The organotypic culture of dorsal root ganglia (DRG) of 10–11-day-old chick embryo was used to test neurite-stimulating activity. A cation protein (16,000 Da) has been purified using a combination of extraction of acid-soluble proteins, preparative electrophoresis, and affinity chromatography on heparin-sepharose. This protein is active at 2–4 ng/ml inducing extensive neurite outgrowth in this bioassay compared to the control one. Many properties distinguish this factor from other known neuronotrophic factors.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5835c661067ef9fcbce3a7500f6b26b4 https://doi.org/10.1016/0304-3940(87)90161-3 Zobrazit plný text záznamu