The cellular selection between apoptosis and autophagy: roles of vitamin D, glucose and immune response in diabetic nephropathy
| Autor: | Magda Hamzawy, Sarah Ali Abdelhameed Gouda, Heba Samy Shoukry, Mary Attia Morcos, Laila Rashid, Nivin Sharawy |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Blood Glucose Male medicine.medical_specialty Endocrinology Diabetes and Metabolism Cell Apoptosis Biology Kidney Diabetes Mellitus Experimental Diabetic nephropathy 03 medical and health sciences 0302 clinical medicine Endocrinology Immune system Downregulation and upregulation Calcitriol Internal medicine Diabetes mellitus medicine Vitamin D and neurology Autophagy Animals Diabetic Nephropathies Rats Wistar Vitamin D Cell Cycle Checkpoints medicine.disease Rats Genes cdc 030104 developmental biology medicine.anatomical_structure Glucose 030220 oncology & carcinogenesis Cytokines Apoptosis Regulatory Proteins |
| Zdroj: | Endocrine. 58(1) |
| ISSN: | 1559-0100 |
| Popis: | Apoptosis, autophagy and cell cycle arrest are cellular responses to injury which are supposed to play fundamental roles in initiation and progression of diabetic nephropathy (DN). The aims of the present study is to shed light on the potential effects of vitamin D analog 22-oxacalcitriol (OCT) on different cell responses during DN, and the possible interplay between both glucose, immune system and vitamin D in determining the cell fate. All rats were randomly allocated into one of three groups: control, vehicle-treated DN group and OCT-treated DN group. Eight weeks after induction of diabetes, the rats were killed. Fasting blood glucose levels, serum 25 (OH) D, renal functions, cytokines and gene expression of autophagy, apoptotic and cell cycle arrest markers were assessed. In addition, the histological assessment of renal architecture was done. OCT treatment remarkably improved the renal functions and albuminuria. The reductions in mesangial cell hypertrophy, extracellular matrix as well as cell loss were significantly associated with upregulation of pro-autophagy gene expressions and downregulation of both pro-apoptotic and G1-cell cycle arrest genes expression. The reno-protective effects of OCT treatment were associated with significant attenuation of the fasting blood glucose, serum IL-6, renal TLR-4 and IFN-g gene expression. Modulator effects of OCT on glucose and immune system play important roles in renal cell fate decision and chronic kidney disease progression. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink