Localized expression of antimicrobial proteins mitigates huanglongbing symptoms in Mexican lime
| Autor: | Oscar Morales-Galván, José-Abrahán Ramírez-Pool, Francisco-Arturo Ramírez-Ortega, Jesús Hinojosa-Moya, Emiliano Loeza-Kuk, José-Abel López-Buenfil, Beatriz Xoconostle-Cázares, Miguel-Angel Guerra-Lupián, Claudio Chavarin-Palacio, Roberto Ruiz-Medrano |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Citrus Agrobacterium Recombinant Fusion Proteins Diaphorina citri Antimicrobial peptides Bioengineering 01 natural sciences Applied Microbiology and Biotechnology Microbiology Defensins 03 medical and health sciences Rhizobiaceae Sieve tube element Vascular tissue Plant Diseases Plant Proteins biology fungi food and beverages General Medicine biology.organism_classification Antimicrobial 030104 developmental biology Muramidase Citrus greening disease Phloem 010606 plant biology & botany Biotechnology |
| Zdroj: | Journal of Biotechnology. 285:74-83 |
| ISSN: | 0168-1656 |
| DOI: | 10.1016/j.jbiotec.2018.08.012 |
| Popis: | Citrus huanglongbing (HLB) is a devastating disease associated with Candidatus Liberibacter asiaticus spp. (CLas), a bacterium restricted to the sieve tube system of the phloem that is transmitted by the psyllid vector, Diaphorina citri. In this study, the human antimicrobial peptides, lysozyme and β-defensin 2, were targeted to the vascular tissue of Mexican lime (Citrus x aurantifolia [Christm.] Swingle) by fusion to a phloem-restricted protein. Localized expression was achieved, via Agrobacterium tumefaciens-mediated transformation of the stem, which led to protein expression and mobilization within the vascular tissue of heterotrophic tissues. HLB-infected plants were monitored for 360 days. Lower bacteria titers were observed in plants expressing either β-defensin 2, lysozyme, or the combination thereof, and these plants had increased photosynthesis, compared to untreated control trees. Thus, targeting of antimicrobial proteins to the vascular tissue was effective in decreasing CLas titer, and alleviating citrus greening symptoms. Based on these findings, this strategy could be used to effectively treat plants that are already infected with bacterial pathogens that reside in the phloem translocation stream. |
| Databáze: | OpenAIRE |
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