Analysis of physiological and miRNA responses to Pi deficiency in alfalfa (Medicago sativa L.)
| Autor: | Hongyu Xu, Cao Jing, Wang Yufei, Tong Zongyong, Zhensong Li, Xianglin Li, Xiufu Wan, Ma Zhao, Zhenyi Li, Li Yue, Liqiang Wan, Feng He |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Plant Science Biology 01 natural sciences Models Biological Plant Roots Phosphates 03 medical and health sciences Auxin Gene Expression Regulation Plant Gene expression Genetics Citric acid secretion KEGG Gene Conserved Sequence chemistry.chemical_classification Base Sequence Gene Expression Profiling fungi food and beverages High-Throughput Nucleotide Sequencing Reproducibility of Results General Medicine Amino acid Metabolic pathway MicroRNAs 030104 developmental biology Gene Ontology Biochemistry Signal transducer activity chemistry RNA Plant Agronomy and Crop Science Plant Shoots 010606 plant biology & botany Medicago sativa |
| Zdroj: | Plant molecular biology. 96(4-5) |
| ISSN: | 1573-5028 |
| Popis: | The induction of miR399 and miR398 and the inhibition of miR156, miR159, miR160, miR171, miR2111, and miR2643 were observed under Pi deficiency in alfalfa. The miRNA-mediated genes involved in basic metabolic process, root and shoot development, stress response and Pi uptake. Inorganic phosphate (Pi) deficiency is known to be a limiting factor in plant development and growth. However, the underlying miRNAs associated with the Pi deficiency-responsive mechanism in alfalfa are unclear. To elucidate the molecular mechanism at the miRNA level, we constructed four small RNA (sRNA) libraries from the roots and shoots of alfalfa grown under normal or Pi-deficient conditions. In the present study, alfalfa plants showed reductions in biomass, photosynthesis, and Pi content and increases in their root-to-shoot ratio and citric, malic, and succinic acid contents under Pi limitation. Sequencing results identified 47 and 44 differentially expressed miRNAs in the roots and shoots, respectively. Furthermore, 909 potential target genes were predicted, and some targets were validated by RLM-RACE assays. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed prominent enrichment in signal transducer activity, binding and basic metabolic pathways for carbohydrates, fatty acids and amino acids; cellular response to hormone stimulus and response to auxin pathways were also enriched. qPCR results verified that the differentially expressed miRNA profile was consistent with sequencing results, and putative target genes exhibited opposite expression patterns. In this study, the miRNAs associated with the response to Pi limitation in alfalfa were identified. In addition, there was an enrichment of miRNA-targeted genes involved in biological regulatory processes such as basic metabolic pathways, root and shoot development, stress response, Pi transportation and citric acid secretion. |
| Databáze: | OpenAIRE |
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