Aptamer RA36 Inhibits of Human, Rabbit, and Rat Plasma Coagulation Activated with Thrombin or Snake Venom Coagulases
| Autor: | A. V. Babij, E. Yu. Savchik, E. N. Lapsheva, T. B. Kalinina, Galina Pavlova, N. N. Drozd, Elena Zavyalova, V. A. Makarov, Alexey Kopylov, Andrey V. Golovin, N. N. Mudrik |
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| Rok vydání: | 2013 |
| Předmět: |
Coagulase
Aptamer Drug Evaluation Preclinical Venom Reptilian Proteins Viper Venoms Thrombin time General Biochemistry Genetics and Molecular Biology Thrombin medicine Animals Humans Blood Coagulation medicine.diagnostic_test Chemistry Anticoagulants General Medicine Aptamers Nucleotide Molecular biology Rats Coagulation Clotting time Snake venom Rabbits medicine.drug |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 156:44-48 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/s10517-013-2274-2 |
| Popis: | RA36 DNA aptamer is a direct anticoagulant prolonging clotting time of human, rabbit, and rat plasma in the thrombin time test. Anticoagulant activity of RA36 is lower than that of recombinant hirudin. During inhibition of human plasma clotting activated with echitox (coagulase from Echis multisquamatus venom), the aptamer presumably binds to meisothrombin exosite I. The sensitivity of human plasma to the aptamer 5-fold surpasses that of rat plasma. Analysis of RA36 binding to coagulase of Agkistrodon halys venom (ancistron) is required for proving the effect of aptamer on polymerization of human fibrinogen. |
| Databáze: | OpenAIRE |
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