A Screen for Short-Range Paracrine Interactions
| Autor: | Katrina H. Spencer, Elliot E. Hui, Monica Y. Kim, Christopher C.W. Hughes |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
General Science & Technology
Cell Biophysics Paracrine Communication Biology Biochemistry Article Cell Line Paracrine signalling Medicinal and Biomolecular Chemistry Cell Line Tumor Gene expression medicine Genetics Humans Telomerase Tumor Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Pharmacology and Pharmaceutical Sciences Fibroblasts Molecular biology In vitro Coculture Techniques Cell biology Gene expression profiling Crosstalk (biology) medicine.anatomical_structure Cell culture Biochemistry and Cell Biology Biotechnology |
| Zdroj: | Integrative biology : quantitative biosciences from nano to macro, vol 6, iss 4 |
| Popis: | Conventional methods for studying paracrine signaling in vitro may not be sensitive to short-range effects resulting from signal dilution or decay. We employ a microfabricated culture substrate to maintain two cell populations in microscale proximity. Individual populations can be quickly retrieved for cell-specific readouts by standard high-throughput assays. We show that this platform is sensitive to short-range interactions that are not detectable by common methods such as conditioned media transfer or porous cell culture inserts, as revealed by gene expression changes in a tumor–stromal crosstalk model. In addition, we are able to detect population-specific gene expression changes that would have been masked in mixed co-cultures. We thus demonstrate a tool for investigating an important class of intercellular communication that may be overlooked in conventional biological studies. |
| Databáze: | OpenAIRE |
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