| Autor: |
Bartsch, Lorenz, Schroeder, Michael P., Hänzelmann, Sonja, Bastian, Lorenz, Lázaro-Navarro, Juan, Schlee, Cornelia, Tanchez, Jutta Ortiz, Schulze, Veronika, Isaakidis, Konstandina, Rieger, Michael A., Gökbuget, Nicola, Eckert, Cornelia, Serve, Hubert, Horstmann, Martin, Schrappe, Martin, Brüggemann, Monika, Baldus, Claudia D., Neumann, Martin |
| Rok vydání: |
2022 |
| DOI: |
10.6084/m9.figshare.19613725 |
| Popis: |
Additional file 7. Validation of CYB5Aalt in patient samples via RT-PCR and Sanger Sequencing. A RT-PCR with specific primers reaching from alternative Exon 1 to Exon 2 of CYB5A WT confirms expression of CYB5Aalt in cDNA derived from two representative patient RNA samples (AL03 REL, AE02 REL). CXCR4 expression was used as positive control, NTC = non-template control. Size of PCR product is shown in bp. Full length electrophoretic gel is shown in Additional File 16. B Sanger sequencing of CYB5Aalt PCR products in (A), depicted as chromatograms, confirms RNAseq sequence (top) of alternative transcript. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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