Simultaneous determination of cysteine sulfinic acid and cysteic acid in rat brain by high-performance liquid chromatography
| Autor: | Kinya Kuriyama, Shuji Ida |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Male
Biophysics Cysteic acid Biochemistry High-performance liquid chromatography chemistry.chemical_compound Animals Cysteine Amino Acids Derivatization Molecular Biology Chromatography High Pressure Liquid Cysteic Acid Brain Chemistry chemistry.chemical_classification Neurotransmitter Agents Chromatography Ion exchange Rats Inbred Strains Cell Biology Rat brain Fluorescence Rats Amino acid Amino Acids Sulfur chemistry Cysteine sulfinic acid |
| Zdroj: | Analytical Biochemistry. 130:95-101 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(83)90654-1 |
| Popis: | A sensitive and specific assay method for cysteine sulfinic acid (CSA) and cysteic acid (CA) using high-performance liquid chromatography has been developed. The method includes post-column derivatization of various amino acids with o-phthalaldehyde in the presence of 2-mercaptoethanol. The column packed with cation-exchange resin (ISC-07/S1504, Shimadzu Scientific Instruments, Inc., Kyoto, Japan) was used for obtaining general separation of amino acids except CSA and CA, while the separation of CSA and CA was achieved using a strong-base anion exchange (ISA-07/S2504, Shimadzu Scientific Instruments) column. The fluorescence peak area for CSA was linear between 20 pmol and 5 nmol, whereas that for CA was 10 pmol to 5 nmol. The regional distribution of CSA, CA, and other amino acids in the rat brain was studied using this new assay method. |
| Databáze: | OpenAIRE |
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