A G-protein-coupled chemokine receptor: A putative insertion site for a multi-pathogen recombinant capripoxvirus vaccine strategy

Autor: Simon Dickmu, Olivier Kwiatek, Catherine Cetre-Sossah, Emmanuel Albina
Přispěvatelé: Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), Laboratoire National Vétérinaire, French Ministry of European and Foreign Affairs (FSP project no. 2003-24 'LABOVET'), Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)
Rok vydání: 2017
Předmět:
0301 basic medicine
Kinase
Virologie
Lumpy Skin Disease
[SDV]Life Sciences [q-bio]
L73 - Maladies des animaux
Antibodies
Viral
New insertion site
Genome
Capripoxvirus
Receptors
G-Protein-Coupled
law.invention
Chemokine receptor
law
Peste des petits ruminants
Chlorocebus aethiops
Immunology and Allergy
vecteur de virus
Expérimentation
Vaccines
Synthetic
biology
Goats
goat
3. Good health
Lumpy skin disease virus
Recombinant DNA
Receptors
Chemokine
stratégie vaccinale
Ovin
Virus Cultivation
Injections
Subcutaneous
Genetic Vectors
030106 microbiology
Immunology
Hemagglutinins
Viral
Hemagglutinin (influenza)
Peste-des-petits-ruminants virus
Viral vector
03 medical and health sciences
Peste-des-Petits-Ruminants
Animals
caprin
Vero Cells
Gene
Génie génétique
Étude de cas
Viral Vaccines
capripoxviridae
biology.organism_classification
Virology
Molecular biology
Mutagenesis
Insertional
030104 developmental biology
Thymidine kinase
Vaccin synthétique
biology.protein
Cattle
Vaccine
Virose
Thymidine
Zdroj: Journal of Immunological Methods
Journal of Immunological Methods, Elsevier, 2017, 448, pp.112-115. ⟨10.1016/j.jim.2017.05.007⟩
ISSN: 0022-1759
DOI: 10.1016/j.jim.2017.05.007
Popis: Capripoxviruses (CaPVs) have been shown to be ideal viral vectors for the development of recombinant multivalent vaccines to enable delivery of immunogenic genes from ruminant pathogens. So far, the viral thymidine kinase (TK) gene is the only gene used to generate recombinants. A putative non-essential gene encoding a G-protein-coupled chemokine receptor subfamily homologue (GPCR) was targeted as an additional insertion site. Peste des petits ruminants (PPR) was chosen as a disease model. A new recombinant CaPV expressing the viral attachment hemagglutinin (H) of the PPR virus (PPRV) in the GPCR insertion site (rKS1-HPPR-GPCR) was generated in the backbone North African isolate KS1 strain of lumpy skin disease virus (LSDV). Comparison with the recombinant CaPV expressing the H of PPRV in the TK gene (rKS1-HPPR-TK) shown to induce protection against both PPR and LSD in both sheep and goats was assessed. The suitability of the GPCR gene to be a putative additional insertion site in the CaPV genome is evaluated and discussed.
Databáze: OpenAIRE
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