Mycoplasma bovis mbfN Encodes a Novel LRR Lipoprotein That Undergoes Proteolytic Processing and Binds Host Extracellular Matrix Components
Autor: | Filimon Mitiku, Fiona M. Sansom, Marc S. Marenda, Kelly A. Tivendale, James Y. Adamu, Philip F. Markham, Glenn F. Browning, Carol A. Hartley |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
Mycoplasma bovis
040301 veterinary sciences Sequence analysis Lipoproteins Blotting Western Virulence Biology Microbiology 0403 veterinary science Rats Sprague-Dawley 03 medical and health sciences Bacterial Proteins Animals Mycoplasma Infections Amino Acid Sequence Adhesins Bacterial Molecular Biology Pathogen Gene Peptide sequence 030304 developmental biology 0303 health sciences Base Sequence Computational Biology 04 agricultural and veterinary sciences Ruminants Extracellular Matrix Fibronectins Rats Bacterial adhesin Models Structural Fibronectin binding Proteolysis Extracellular matrix binding Cattle Electrophoresis Polyacrylamide Gel Sequence Alignment Research Article |
Zdroj: | J Bacteriol |
Popis: | Mycoplasma bovis causes serious infections in ruminants, leading to huge economic losses. Lipoproteins are key components of the mycoplasma membrane and are believed to function in nutrient acquisition, adherence, enzymatic interactions with the host, and induction of the host’s immune response to infection. Many genes of M. bovis have not been assigned functions, in part because of their low sequence similarity with other bacteria, making it difficult to extrapolate gene functions. This study examined functions of a surface-localized leucine-rich repeat (LRR) lipoprotein encoded by mbfN of M. bovis PG45. Homologs of MbfN were detected as 48-kDa peptides by Western blotting in all the strains of M. bovis included in this study, with the predicted 70-kDa full-length polypeptide detected in some strains. Sequence analysis of the gene revealed the absence in some strains of a region encoding the carboxyl-terminal 147 amino acids found in strain PG45, which could account for the variation detected by immunoblotting. In silico analysis of MbfN suggested that it may have an adhesion-related function. In vitro binding assays confirmed MbfN to be a fibronectin and heparin-binding protein. Disruption of mbfN in M. bovis PG45 significantly reduced (P = 0.033) the adherence of M. bovis PG45 to MDBK cells in vitro, demonstrating the role of MbfN as an adhesin. IMPORTANCE Experimental validation of the putative functions of genes in M. bovis will advance our understanding of the basic biology of this economically important pathogen and is crucial in developing prevention strategies. This study demonstrated the extracellular matrix binding ability of a novel immunogenic lipoprotein of M. bovis, and the role of this protein in adhesion by M. bovis suggests that it could play a role in virulence. |
Databáze: | OpenAIRE |
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