Conservation of biological properties of the CD40 ligand, CD154 in a non-mammalian vertebrate
| Autor: | Harrie L. Glansbeek, Joan Burnside, John R. Young, Andrew C. Gill, Lawrence G. Hunt, Clive A. Tregaskes |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
medicine.drug_class
CD8 Antigens Recombinant Fusion Proteins CD40 Ligand Molecular Sequence Data Immunology Monoclonal antibody Evolution Molecular Mice Complementary DNA medicine Animals Amino Acid Sequence CD154 Receptor biology Antibodies Monoclonal Germinal center Fusion protein Molecular biology Cell biology biology.protein Cattle Tumor necrosis factor alpha Antibody Chickens Developmental Biology |
| Zdroj: | Tregaskes, C A, Glansbeek, H L, Gill, A C, Hunt, L G, Burnside, J & Young, J R 2005, ' Conservation of biological properties of the CD40 ligand, CD154 in a non-mammalian vertebrate ', Developmental and Comparative Immunology, vol. 29, no. 4, pp. 361-374 . https://doi.org/10.1016/j.dci.2004.09.001 |
| ISSN: | 0145-305X |
| Popis: | Signals delivered by the CD40 ligand, CD154, have crucial roles in immune responses in mammals, being required for development of germinal centres, maturation of T-dependent antibody responses, and generation of B-cell memory. To determine whether these functions were conserved in a non-mammalian species, a putative chicken CD154 cDNA was used to make an oligomeric fusion protein, and to raise monoclonal antibodies. The antibodies detected surface expression on activated T-cells. The fusion protein detected expression of a receptor on B-cells, thrombocytes and macrophages. Biological effects of the fusion protein included induction of NO synthesis in a macrophage cell line, enhancement of splenic B-cell survival, and induction of apoptosis in a bursal lymphoma cell line. These observations demonstrated substantial functional equivalence with mammalian CD154 and thus provided evidence for the early evolutionary emergence of the set of functions associated with this molecule, and its central role in the vertebrate immune system. (C) 2004 Elsevier Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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