Comparative transcriptional analysis for Toll-like receptors, inflammatory cytokines, and apoptotic genes in response to different cytolethal-encoding and noncoding isolates of Salmonella enterica and Campylobacter jejuni from food and human stool
| Autor: | Mohammad Hassan Shirazi, Mohammad Reza Zali, Leila Ganji, Masoud Alebouyeh |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Diarrhea
0301 basic medicine Salmonella Bacterial Toxins 030106 microbiology Apoptosis Clostridium difficile toxin B medicine.disease_cause Microbiology Campylobacter jejuni Proinflammatory cytokine Feces 03 medical and health sciences Bacterial Proteins Campylobacter Infections medicine Humans RNA Messenger Inflammation biology Caspase 3 Tumor Necrosis Factor-alpha Campylobacter Interleukin-8 Toll-Like Receptors Salmonella enterica Epithelial Cells biology.organism_classification Toll-Like Receptor 2 Up-Regulation Toll-Like Receptor 4 Toll-Like Receptor 5 TLR2 030104 developmental biology Infectious Diseases TLR5 Food Microbiology Cytokines Transcriptome HT29 Cells |
| Zdroj: | Microbial Pathogenesis. 133:103550 |
| ISSN: | 0882-4010 |
| Popis: | Diversity of Campylobacter and Salmonella strains in interaction with epithelial cells may explain distinct modes of the pathogenesis, varying from mild watery to severe inflammatory diarrhea. We analyzed impact of this diversity, in relation to carriage and expression of cytholethal distending toxin B (cdtB), on alteration of IL-8, TNF-α, TLR2, TLR4, TLR5, CASP3 mRNA and cytokine levels in HT-29 cell line. A diversity was observed for induction of genes among different strains. Great diversity in IL-8 induction was detected between cdtB+ and cdtB− strains. Early analysis showed down-regulation of TNF-α, mostly among cdtB+ strains. Any increase or decrease in expression of TLR2 in the cdtB− C. jejuni strains was orderly correlated with increase or decrease of TLR4 and TNF-α. Up-regulation of CASP3 was followed by upregulation of TLR2, -4 and/or TNF-α, regardless to the cdtB status. In conclusion, induction of inflammatory response could mediate by distinct C. jejuni and S. enterica strains by several ways. |
| Databáze: | OpenAIRE |
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