Quality and fertility of extended boar semen after prolonged transport
| Autor: | Aleksandar Milovanović, Miloš Pavlović, Marina Lazarević, Aleksandar Masic, Jelena Apić, Tomislav Barna, Nevena Maksimović |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
endocrine system
Computer-assisted sperm analyses (CASA) General Veterinary 040301 veterinary sciences urogenital system media_common.quotation_subject 0402 animal and dairy science Boars Fertility 04 agricultural and veterinary sciences Biology 040201 dairy & animal science 0403 veterinary science Boar semen Animal science Import Semen Import semen boars computer-assisted sperm analyses (CASA) flow cytometry Quality (business) Flow cytometry media_common |
| Zdroj: | Turkish Journal of Veterinary and Animal Sciences |
| Popis: | The aim of this study was to analyse semen quality parameters of fresh-extended porcine semen imported from Denmark through prolonged importing regulations and transport and to analyse fertility performance in sows inseminated four days post semen collection. Semen was analysed immediately upon the arrival (4 days post collection) using computer-assisted sperm analyses (CASA), cytomorphology and flow cytometry, and sows were inseminated later the same day. The mean sperm concentration was 1718.09 ± 100.11 × 106, with average of 1193.40 ± 90.58 × 106 motile cells (69%) and 756.04 ± 70.57 × 106 progressive motile spermatozoa (43.58%) in a dose, which was almost twice lower in value than producer certified-declared number of 2 billion of motile cells. Acrosome and sperm membrane integrity test indicated high percentage of total damaged acrosome. Mean values of semen samples analysed by sperm chromatin structure assay (SCSA) showed relatively high degree of chromatin damage. Membrane permeability test showed high percentage of sperm with damaged membrane. Prolonged transport negatively affected the sperm quality in terms of both motility and chromatin structure stability. Fertility of sows was affected by semen quality in terms of concentration and sperm motility parameters, as well as ratio of live sperm cells population with damaged acrosome. © 2021 TÜBİTAK. |
| Databáze: | OpenAIRE |
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