Opioid receptors on bone marrow neutrophils modulate chemotaxis and CD11b/CD18 expression
| Autor: | Bruce Walcheck, Anjali Kulkarni-Narla, David R. Brown |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
Agonist medicine.medical_specialty Enkephalin Neutrophils medicine.drug_class Narcotic Antagonists Chemokine CXCL2 Macrophage-1 Antigen Bone Marrow Cells (+)-Naloxone Mice chemistry.chemical_compound Internal medicine medicine Animals Receptor Pharmacology Dose-Response Relationship Drug biology Naloxone Chemotaxis Receptors Opioid kappa 3 4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide (trans)-Isomer Analgesics Non-Narcotic Molecular biology Mice Inbred C57BL Endocrinology Mechanism of action Integrin alpha M chemistry CD18 Antigens biology.protein Bremazocine Chemokines medicine.symptom |
| Zdroj: | European Journal of Pharmacology. 414:289-294 |
| ISSN: | 0014-2999 |
| DOI: | 10.1016/s0014-2999(01)00727-0 |
| Popis: | Opiates impair neutrophil-mediated host defense, but the involvement of κ-opioid receptors in this action has not been defined. The selective κ-opioid receptor agonist [ trans -(+)3,4-dichloro- N -methyl- N [2-(1-pyrrolidinyl)-cyclohexyl]benzeneacetamide methanesulfonate inhibited macrophage inflammatory protein-2-induced chemotaxis of bone marrow neutrophils from C57BL/6 mice. Its effects were concentration-dependent ( p IC 50 =10.40±0.61) and inhibited by naloxone ( K e =0.27 nM). The κ-opioid receptor agonists bremazocine and ICI-204, 488 also inhibited chemotaxis, as did the respective μ- and δ-opioid receptor agonists [ d -Ala 2 , N -methyl-Phe 4 , Gly 5 -ol]enkephalin and [ d -Pen 2,5 ]enkephalin albeit with lower potencies. U-50,488H also decreased neutrophil expression of the β 2 integrin CD11b/CD18 (Mac-1) and adhesion to plastic in a naloxone-reversible manner. The results indicate that κ-opioid receptors expressed by neutrophils rapidly modulate chemotaxis and adhesion in vitro. |
| Databáze: | OpenAIRE |
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