Development of cross-protective Eimeria-vectored vaccines based on apical membrane antigens
| Autor: | Virginia Marugán-Hernández, Karen J. Billington, Sungwon Kim, Damer P. Blake, Janene M. Bumstead, David J. P. Ferguson, Iván Pastor-Fernández, Lonneke Vervelde, Fiona M. Tomley, Tatiana Küster |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Protozoan Vaccines
0301 basic medicine Transgene 030231 tropical medicine Heterologous Antigens Protozoan Eimeria DNA vaccination 03 medical and health sciences 0302 clinical medicine Antigen parasitic diseases Cross protection Animals Poultry Diseases biology Heterologous protection Coccidiosis Apical membrane Apical membrane antigen biology.organism_classification Virology Specific Pathogen-Free Organisms 3. Good health Vaccination 030104 developmental biology Infectious Diseases Eimeria maxima Vaccine delivery vector AMA1 AMA2 Parasitology Chickens Eimeria tenella |
| Zdroj: | International Journal for Parasitology Pastor-Fernández, I, Kim, S, Billington, K, Bumstead, J, Marugán-Hernández, V, Küster, T, Ferguson, D J P, Vervelde, L, Blake, D P & Tomley, F M 2018, ' Development of cross-protective Eimeria-vectored vaccines based on apical membrane antigens ', International Journal For Parasitology . https://doi.org/10.1016/j.ijpara.2018.01.003 |
| DOI: | 10.1016/j.ijpara.2018.01.003 |
| Popis: | Recently, the availability of protocols supporting genetic complementation of Eimeria has raised the prospect of generating transgenic parasite lines which can function as vaccine vectors, expressing and delivering heterologous proteins. Complementation with sequences encoding immunoprotective antigens from other Eimeria spp. offers an opportunity to reduce the complexity of species/strains in anticoccidial vaccines. Herein, we characterise and evaluate EtAMA1 and EtAMA2, two members of the apical membrane antigen family of parasite surface proteins from Eimeria tenella. Both proteins are stage-regulated, and the sporozoite-specific EtAMA1 is effective at inducing partial protection against homologous challenge with E. tenella when used as a recombinant protein vaccine, whereas the merozoite-specific EtAMA2 is not. In order to test the ability of transgenic parasites to confer heterologous protection, E. tenella parasites were complemented with EmAMA1, the sporozoite-specific orthologue of EtAMA1 from E. maxima, coupled with different delivery signals to modify its trafficking and improve antigen exposure to the host immune system. Vaccination of chickens using these transgenic parasites conferred partial protection against E. maxima challenge, with levels of efficacy comparable to those obtained using recombinant protein or DNA vaccines. In the present work we provide evidence for the first known time of the ability of transgenic Eimeria to induce cross protection against different Eimeria spp. Genetically complemented Eimeria provide a powerful tool to streamline the complex multi-valent anticoccidial vaccine formulations that are currently available in the market by generating parasite lines expressing vaccine targets from multiple eimerian species. |
| Databáze: | OpenAIRE |
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