Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) clones from Paraguayan children
| Autor: | Juana Ortellado, Mónica Rodríguez, Ana Campuzano, Carmen Espínola, Héctor Escobar Castro, Silvina Fernández, Rocio Arguello, Gloria Gómez, Lorena Grau, Marta Mollerach, Sol Haim, Gladys Velázquez, Beatriz Quiñónez, Patricia Almada, Rosa Guillén, Gloria Samudio, Fátima Rodríguez, Wilma Basualdo, Claudia Salinas |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Methicillin-Resistant Staphylococcus aureus medicine.medical_specialty Adolescent Virulence Factors 030106 microbiology Child Health Services Biology Multiple Loci VNTR Analysis medicine.disease_cause Microbiology Community associated 03 medical and health sciences Virology Epidemiology medicine Pulsed-field gel electrophoresis Humans Genetic variability Typing Child Infant Newborn Infant General Medicine Staphylococcal Infections Methicillin-resistant Staphylococcus aureus Anti-Bacterial Agents Electrophoresis Gel Pulsed-Field Community-Acquired Infections 030104 developmental biology Infectious Diseases Cross-Sectional Studies Staphylococcus aureus Paraguay Child Preschool Parasitology Female Multilocus Sequence Typing |
| Zdroj: | Journal of infection in developing countries. 14(3) |
| ISSN: | 1972-2680 |
| Popis: | Introduction: Staphylococcus aureus is considered one of the most important human pathogens, and its levels of resistance to methicillin have increased even in strains isolated from people without nosocomial risk factors. Molecular analysis is essential for understanding the patterns of dissemination. The objective of this study was to identify community-acquired methicillin-resistant S. aureus (CA-MRSA) clones that infected Paraguayan children patients in two periods of time. Methodology: An observational, descriptive study was designed to determine the genetic variability of 115 isolates of CA-MRSA recovered from children who attended four reference centers in Paraguay between 2009-2010 and 2012-2013. Results: The combined use of Pulsed Field Gel Electrophoresis (PFGE), Multi-Locus Sequencing Typing, Multi-Locus Variable Analysis (MLVA) and Spa typing techniques allowed the identification of two dominant clones: ST30-IV-t019 (77%) and ST5-IV-t311 (10%), and the establishment of the former as the leading cause of CA-MRSA infections in children during the study period. Conclusions: This is the first study that provides epidemiological information as well as microbiological and molecular characteristics of CA-MRSA isolates recovered from children from Asunción and the Central Department of Paraguay. |
| Databáze: | OpenAIRE |
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