Assessment of Anti-Tumor potential and safety of application of Glutathione stabilized Gold Nanoparticles conjugated with Chemotherapeutics
| Autor: | Ewelina Barcińska, Katarzyna Sobczak, Karol P Steckiewicz, Michal Wojcik, Ewelina Tomczyk, Iwona Inkielewicz-Stepniak |
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| Rok vydání: | 2019 |
| Předmět: |
Metal Nanoparticles
Conjugated system Gold nanoparticles conjugate Deoxycytidine 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Microscopy Electron Transmission Pancreatic cancer Neoplasms medicine Cytotoxic T cell Humans Gold nanoparticles Doxorubicin MTT assay Telomerase Chromatography High Pressure Liquid Drug deliver platform Cancer Chemotherapeutic Osteoblasts Cytarabine General Medicine Glutathione medicine.disease Gemcitabine chemistry Colloidal gold Cancer research MCF-7 Cells 030211 gastroenterology & hepatology Gold medicine.drug Research Paper |
| Zdroj: | International Journal of Medical Sciences |
| ISSN: | 1449-1907 |
| Popis: | Due to the high toxicity of currently used chemotherapeutics, novel methods of cancer treatment are needed. Gold nanoparticles (AuNPs) seem to be an interesting alternative due to penetration through biological membranes and systemic barriers. AuNPs as carriers of chemotherapeutics allow for reduced concentrations whilst maintaining the expected effect, and thus reducing the costs of therapy and adverse effects. We synthesized AuNPs stabilized with reduced glutathione (GSH) and conjugated with doxorubicin (DOX), gemcitabine (GEM) or cytarabine (CTA). This is the first study in which cytarabine-AuNPs were synthesized and characterized. Transmission electron microscopy (TEM), thermogravimetric analysis (TGA), nuclear magnetic resonance spectroscopy (NMR) and high-performance liquid chromatography (HPLC) were used to chemically characterize obtained nanoparticles. Antitumor activity and safety of application were assessed by MTT assay in in vitro model (human osteosarcoma cells -143B, human osteoblast- hFOB1.19, breast cancer cells - MCF7, breast epithelial cells - MCF10A, pancreatic cancer cells - PANC-1, and pancreatic cells - hTERT-HPNE cells). We have shown that cellular response varies according to the type and concentration of AuNPs. At some concentrations, we were able to show selective cytotoxicity of our AuNPs conjugates only to cancer cell lines. Synthesized nanoparticles were more cytotoxic to tumor cell lines than chemotherapeutics alone. |
| Databáze: | OpenAIRE |
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