Novel fluo-4 analogs for fluorescent calcium measurements
| Autor: | Vladimir Martin, Kyle R. Gee, Josh Morgan, Anca Rothe, Michael Beierlein |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Streptavidin
Retinal Ganglion Cells Physiology chemistry.chemical_element Calcium Calcium Measurement chemistry.chemical_compound BAPTA Cerebellum Moiety Animals Molecular Biology Fluorescent Dyes Fluo-4 Aniline Compounds Chemistry Cell Biology Fluorescence Rats Biochemistry Xanthenes Biotinylation cardiovascular system Biophysics Chickens |
| Zdroj: | Cell calcium. 36(6) |
| ISSN: | 0143-4160 |
| Popis: | We report new fluorescent calcium indicators based on fluo-4. Attachment of a carboxamide or methylenecarboxamide moiety to the BAPTA chelator portion of fluo-4 allowed for the attachment of dextrans, protein-reactive moieties, and biotin. In particular, a high affinity fluo-4 dextran conjugate was prepared and shown to be functional in brain slices. All new probes were characterized spectroscopically and exhibited large fluorescence increases upon calcium-binding. The biotinylated version of fluo-4 formed a persistent streptavidin complex which still responded to increasing calcium concentrations with a large fluorescence increase. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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