μ1A-adaptin-deficient mice: lethality, loss of AP-1 binding and rerouting of mannose 6-phosphate receptors
| Autor: | Daniela Zizioli, Eeva-Liisa Eskelinen, Jens Hamann, Paul Saftig, Peter Schu, Kurt von Figura, Susanne Lausmann, Christoph Meyer |
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| Rok vydání: | 2000 |
| Předmět: |
Endosome
Clathrin adaptor complex Adaptor Protein Complex 1 Mannose Mannose 6-phosphate Biology adaptins environment and public health Clathrin Receptor IGF Type 2 General Biochemistry Genetics and Molecular Biology blastocytes Embryonic and Fetal Development Mice chemistry.chemical_compound Adaptor Protein Complex alpha Subunits Animals mouse knock out Receptor Molecular Biology General Immunology and Microbiology General Neuroscience Vesicle Gene Expression Regulation Developmental Membrane Proteins Biological Transport Articles Adaptor Protein Complex mu Subunits Cell biology Adaptor Proteins Vesicular Transport chemistry Cytoplasm biology.protein |
| Zdroj: | The EMBO Journal. 19:2193-2203 |
| ISSN: | 1460-2075 0261-4189 |
| Popis: | The heterotetrameric AP-1 complex is involved in the formation of clathrin-coated vesicles at the trans-Golgi network (TGN) and interacts with sorting signals in the cytoplasmic tails of cargo molecules. Targeted disruption of the mouse mu1A-adaptin gene causes embryonic lethality at day 13.5. In cells deficient in micro1A-adaptin the remaining AP-1 adaptins do not bind to the TGN. Polarized epithelial cells are the only cells of micro1A-adaptin-deficient embryos that show gamma-adaptin binding to membranes, indicating the formation of an epithelial specific AP-1B complex and demonstrating the absence of additional mu1A homologs. Mannose 6-phosphate receptors are cargo molecules that exit the TGN via AP-1-clathrin-coated vesicles. The steady-state distribution of the mannose 6-phosphate receptors MPR46 and MPR300 in mu1A-deficient cells is shifted to endosomes at the expense of the TGN. MPR46 fails to recycle back from the endosome to the TGN, indicating that AP-1 is required for retrograde endosome to TGN transport of the receptor. |
| Databáze: | OpenAIRE |
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