Association between the Genetic Variants of Glutathione Peroxidase 4 and Severity of Endometriosis
| Autor: | Maw-Sheng Lee, Chung-Hsien Liu, Tsung-Hsien Lee, Shun-Fa Yang, Cheng-Hsuan Wu, Po-Hui Wang, Long-Yao Lin, Yun-Yao Huang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
endometriosis
Adult medicine.medical_specialty Genotype Health Toxicology and Mutagenesis Endometriosis lcsh:Medicine Single-nucleotide polymorphism Gastroenterology Polymorphism Single Nucleotide Article Pathogenesis 03 medical and health sciences 0302 clinical medicine single nucleotide polymorphism Internal medicine Medicine SNP oxidative stress Humans Genetic Predisposition to Disease Prospective Studies Allele frequency 030304 developmental biology chemistry.chemical_classification 0303 health sciences TXN2 Glutathione Peroxidase business.industry Glutathione peroxidase lcsh:R Public Health Environmental and Occupational Health thioredoxin reductase thioredoxin medicine.disease Phospholipid Hydroperoxide Glutathione Peroxidase chemistry 030220 oncology & carcinogenesis Case-Control Studies Female Thioredoxin business |
| Zdroj: | International Journal of Environmental Research and Public Health International Journal of Environmental Research and Public Health, Vol 17, Iss 5089, p 5089 (2020) Volume 17 Issue 14 |
| ISSN: | 1660-4601 1661-7827 |
| Popis: | It has been reported that oxidative and nitrative stress might be the pathogenesis of endometriosis. This prospective case-control study attempted to check the connection between single nucleotide polymorphism (SNP) of three antioxidant enzymes (glutathione peroxidase 4 (GPX4), thioredoxin 2 (TXN2), thioredoxin reductase 1 (TXNRD1)) and endometriosis. We recruited 90 patients with histology-approved endometriosis as the case group and 130 age-matched women for an annual pap smear examination as the control group. The stage of endometriosis was evaluated with revised ASRM score. Both groups were genotyped in the peripheral leukocytes for the SNP of GPX4 (rs713041), TXN2 (rs4821494) and TXNRD1 (rs1128446) by PCR-based methods. An X2 test was used to analysis of the difference of allele frequency and SNP distribution between two groups. The results revealed GPX4 (rs713041) has a significantly different distribution between two groups (C:T = 116 (44.6%):144 (55.4%) in control and C:T = 104 (57.8%): 76 (42.2%) in endometriosis groups, p = 0.007). The SNP in TXN2 (rs4821494) also showed a difference in allele frequency (G:T = 180 (69.2%):80 (30.8%) in control and G:T = 141 (78.3%):39 (21.6%) in endometriosis group, p = 0.030). In addition, the SNP GPX4 (rs713041) was associated with the severity of the endometriosis. Women who have advanced stage endometriosis were different from mild endometriosis in genetic variants of GPX4 gene (p = 0.001). In conclusion, the relationship between endometriosis and SNP of antioxidant enzymes, GPX4 and TXN2, was confirmed by the present study. According to the result, we suggested that the GPX4 might contribute to the pathogenesis of endometriosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|