Noninvasive assessment of alveolar microvascular recruitment in conscious non-sedated rats
| Autor: | Priya Ravikumar, Connie C. W. Hsia, Roger H Unger, Dan M. Dane, Cuneyt Yilmaz |
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| Rok vydání: | 2013 |
| Předmět: |
Pulmonary and Respiratory Medicine
Leptin Male medicine.medical_specialty Pulmonary Circulation Consciousness Physiology Article DLCO Diffusing capacity Internal medicine medicine Animals Lung volumes Obesity business.industry Pulmonary Gas Exchange General Neuroscience Pulmonary Diffusing Capacity Microangiopathy Age Factors Oxygenation respiratory system medicine.disease Surgery Capillaries Rats Pulmonary Alveoli Disease Models Animal Cardiology Arterial blood Rats Transgenic business Perfusion |
| Zdroj: | Respiratory physiologyneurobiology. 190 |
| ISSN: | 1878-1519 |
| Popis: | Recruitment of alveolar microvascular reserves, assessed from the relationship between pulmonary diffusing capacity (DLCO) and perfusion (Q˙c), is critical to the maintenance of arterial blood oxygenation. Leptin-resistant ZDF fatty diabetic (fa/fa) rats exhibit restricted cardiopulmonary physiology under anesthesia. To assess alveolar microvascular function in conscious, non-sedated, non-instrumented, and minimally restrained animals, we adapted a rebreathing technique to study fa/fa and control non-diabetic (+/+) rats (4-5 and 7-11mo old) at rest and during mild spontaneous activity. Measurements included O2 uptake, lung volume, Q˙c, DLCO, membrane diffusing capacity (DMCO), capillary blood volume (Vc) and septal tissue-blood volume. In older fa/fa than +/+ animals, DLCO and DMCO at a given Q˙c were lower; Vc was reduced in proportion to Q˙c. Results demonstrate the consequences of alveolar microangiopathy in the metabolic syndrome: lung volume restriction, reduced Q˙c, and elevated membrane resistance to diffusion. At a given Q˙c, DLCO is lower in rats and guinea pigs than dogs or humans, consistent with limited alveolar microvascular reserves in small animals. |
| Databáze: | OpenAIRE |
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