Effects of Nitric Oxide on Aldosterone Synthesis and Nitric Oxide Synthase Activity in Glomerulosa Cells from Bovine Adrenal Gland

Autor: Jose M. Macarulla, María A. Rábano, Miguel Trueba, Zoraida J. Patrignani, Carolina Mondillo, José M. Sainz, Omar Pedro Pignataro, Cecilia Reche
Rok vydání: 2004
Předmět:
Cell Extracts
Male
Nitroprusside
endocrine system
medicine.medical_specialty
Angiotensins
Endocrinology
Diabetes and Metabolism
Nitric Oxide Synthase Type II
Receptors
Cytoplasmic and Nuclear
Stimulation
Nitric oxide
chemistry.chemical_compound
Soluble Guanylyl Cyclase
Endocrinology
Adrenocorticotropic Hormone
Internal medicine
Cyclic AMP
Cyclic GMP-Dependent Protein Kinases
Polyamines
medicine
Animals
Nitric Oxide Donors
Enzyme Inhibitors
Aldosterone
Cyclic GMP
Cells
Cultured
omega-N-Methylarginine
Dose-Response Relationship
Drug
biology
Angiotensin II
Hydroxycholesterols
Nitric oxide synthase
Ethisterone
NG-Nitroarginine Methyl Ester
chemistry
Guanylate Cyclase
biology.protein
Cattle
Zona Glomerulosa
Sodium nitroprusside
Nitric Oxide Synthase
Soluble guanylyl cyclase
cGMP-dependent protein kinase
hormones
hormone substitutes
and hormone antagonists
medicine.drug
Zdroj: Endocrine. 24:061-072
ISSN: 0969-711X
Popis: This study investigated the effects of two NO-releasing agents, diethylenetriamine-NO (deta-NO) and sodium nitroprusside (SNP), on basal, ACTH-, and angiotensin II (AngII)-stimulated aldosterone production in glomerulosa cells from bovine adrenal gland. NO donors inhibited basal and ACTH- or AngII-stimulated aldosterone synthesis in a concentration-dependent manner. Deta-NO and SNP also provoked a concentration-dependent stimulation of cGMP production. However, cGMP was not responsible for the inhibition of aldosterone secretion, because a cGMP analog did not reproduce the inhibitory effect. Moreover, soluble guanylyl cyclase or protein kinase G inhibitors did not revert the inhibitory effect of NO on aldosterone production. NO donors did not modify ACTH-stimulated cAMP production or AngII-stimulated PLC activity stimulation, but inhibited 22[R] hydroxycholesterol- or pregnenolone-stimulated aldosteronogenesis. NO can be synthesized in bovine glomerulosa cells because nitrite production was determined and characterization of NOS activity was also performed. Nitrite accumulation was not modified in the presence of ACTH, AngII, or other factors used to induce iNOS. NOS activity that showed a Michaelis-Menten kinetic was NADPH- and calcium-dependent and was inhibited by two competitive inhibitors, L-NAME and L-NMMA. These results show that NO inhibits aldosterone production in glomerulosa cells acting on P450scc and other P450-dependent steroidogenic enzymes, and these cells display NOS activity suggesting that NO can be produced by constitutive NOS isozymes.
Databáze: OpenAIRE
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