Stereoselective HPLC separation of alvimopan on cellulose-based immobilized polysaccharide as a chiral stationary phase
| Autor: | K. Hima Bindu, Nitin H. Dhekale, Dattatray B. Gunjal, Anil H. Gore, Govind B. Kolekar, Yagnakirankumar Komaravolu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Pharmacology
Active ingredient Diethylamine Ethanol Chromatography 010405 organic chemistry 010401 analytical chemistry Organic Chemistry Isopropyl alcohol 01 natural sciences High-performance liquid chromatography Catalysis 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound chemistry Phase (matter) Drug Discovery Stereoselectivity Cellulose Spectroscopy |
| Zdroj: | Chirality. |
| ISSN: | 1520-636X |
| Popis: | Chiral separation by normal phase high performance liquid chromatography is one of the most powerful technique to quantify the chiral purity of the compounds. In this study, a novel, simple, and specific analytical method was proposed to ascertain the chiral purity of alvimopan (ALV). The normal phase HPLC method was developed based on cellulose tris (3,5-dichlorophenylcarbamate) stationary phase. The separation of ALV isomers achieved by using column CHIRALPAK IC (250 × 4.6 mm, 5 μm), mobile phase n-hexane: isopropyl alcohol: ethanol: diethylamine (650:200:150:5 v/v), column oven temperature 30°C, flow rate 1.0 mL min-1 , injection volume was 10 μL, chromatographic response monitored at 273 nm. The developed method was validated as per the ICH guidelines and found precise, accurate, and linear. The advantage of the method is a good separation of ALV isomers within 35 minutes of the analysis time. Therefore, this method is suitable for routine determination of chiral purity of ALV active pharmaceutical ingredient. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text