DNA Adduct Formation in the Lungs and Brain of Rats Exposed to Low Concentrations of [13C2]-Acetaldehyde
| Autor: | Florencio Porto Freitas, Marisa Helena Gennari de Medeiros, Fernando Silva Lopes, Victor Henrique Carvalho, Angélica B. Sanchez, Graziella E. Ronsein, Camila Carrião Machado Garcia, Paolo Di Mascio, Guilherme Lopes Batista, Ivano Gebhardt Rolf Gutz |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
ADUTOS DE DNA Mutagenesis Acetaldehyde General Medicine Toxicology Tobacco smoke Adduct 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine chemistry Biochemistry 030220 oncology & carcinogenesis Deoxyguanosine DNA Adduct Formation Volume concentration DNA |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1520-5010 0893-228X |
| DOI: | 10.1021/acs.chemrestox.8b00016 |
| Popis: | Air pollution is a major environmental risk for human health. Acetaldehyde is present in tobacco smoke and vehicle exhaust. In this study, we show that [13C2]-acetaldehyde induces DNA modification with the formation of isotopically labeled 1, N2-propano-2'-deoxyguanosine adducts in the brain and lungs of rats exposed to concentrations of acetaldehyde found in the atmosphere of megacities. The adduct, with the addition of two molecules of isotopically labeled acetaldehyde [13C4]-1, N2-propano-dGuo, was detected in the lung and brain tissues of exposed rats by micro-HPLC/MS/MS. Structural confirmation of the products was unequivocally performed by nano-LC/ESI+-HRMS3 analyses. DNA modifications induced by acetaldehyde have been regarded as a key factor in the mechanism of mutagenesis and may be involved in the cancer risks associated with air pollution. |
| Databáze: | OpenAIRE |
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