Angiotensin converting enzyme activity in the serum, lung and kidney of diabetic rats
| Autor: | B. Chen-Gal, Arie Erman, Joseph B. Rosenfeld, D J Van Dyk, Geoffrey Boner, I. D. S. Giler |
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| Rok vydání: | 1993 |
| Předmět: |
Male
medicine.medical_specialty Increased glomerular filtration rate medicine.medical_treatment Clinical Biochemistry Peptidyl-Dipeptidase A Kidney Biochemistry Plasma renin activity Diabetes Mellitus Experimental Internal medicine Diabetes mellitus Renin medicine Animals Insulin Rats Wistar Lung Pancreatic hormone biology business.industry Angiotensin-converting enzyme General Medicine medicine.disease Rats Endocrinology medicine.anatomical_structure biology.protein business |
| Zdroj: | European journal of clinical investigation. 23(10) |
| ISSN: | 0014-2972 |
| Popis: | Diabetes Mellitus in its early stages, is associated with kidney enlargement and increased glomerular filtration rate in humans and in rats. The present study was designed to clarify the direct effect of diabetes on serum and tissue angiotensin converting enzyme (ACE) activity in streptozotocin-induced diabetic rats. Serum ACE activity, as determined using a radiometric assay, was significantly increased in the diabetic rats (n = 15) 14 days after induction of diabetes (670 +/- 31 vs. 506 +/- 14 nmol ml-1 min-1). Lung ACE activity, but not renal, was significantly elevated at 7 and 14 days by 29 and 46%, respectively. Plasma renin activity in the diabetic rats was decreased at 7 and 14 days by 41 and 78%, respectively. Incubations of lung slices in the presence of glucose at different concentrations did not affect in-vitro release of the enzyme. Administration of insulin (8 units kg-1) to diabetic rats (n = 6) on the 4th day for 11 days reduced ACE activity to values below control. Thus, serum and lung ACE activity is increased in the diabetic rat and reduced upon insulin treatment. |
| Databáze: | OpenAIRE |
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