The protein modifier SUMO is critical for integrity of the Arabidopsis shoot apex at warm ambient temperatures
| Autor: | Bas Beerens, Valentin Hammoudi, Martijs J. Jonker, Mark Kwaaitaal, Tieme A Helderman, Georgios Vlachakis, Marcel Giesbers, Harrold A. van den Burg |
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| Přispěvatelé: | SILS Other Research (FNWI), Molecular Plant Pathology (SILS, FNWI), RNA Biology & Applied Bioinformatics (SILS, FNWI) |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Wageningen Electron Microscopy Centre Gene knockdown biology Physiology Chemistry Mutant SUMO protein Plant Science biology.organism_classification 01 natural sciences Phenotype Cell biology Heat shock factor 03 medical and health sciences 030104 developmental biology Degree Celsius Arabidopsis Shoot Life Science 010606 plant biology & botany |
| Zdroj: | Journal of Experimental Botany, 72(21), 7531-7548. Oxford University Press Journal of Experimental Botany, 72(21), 7531-7548 Journal of Experimental Botany 72 (2021) 21 |
| ISSN: | 1460-2431 0022-0957 |
| DOI: | 10.1093/jxb/erab262 |
| Popis: | SUMO is a protein modification whose conjugate levels peak during acute heat stress. Here, we found that SUMO is also critical for plant longevity when Arabidopsis experiences a prolonged non-damaging period of 28 °C. Thermo-lethality at 28 °C was seen in sumo1/2 knockdown mutants but not in any other mutant of the SUMO pathway tested. Autoimmunity due to low SUMO1/2 expression levels was not causal for this thermo-lethality. The role of SUMO in thermo-resilience was also distinct from its requirement for thermomorphogenesis—a growth response triggered by the same warm temperature, as only the latter response was also dependent on the SUMO ligase SIZ1. Thermo-resilience at 28 °C and (acquired) thermotolerance, a response that allows plants to recover and acclimate to brief extreme temperatures, both depend on the HEAT SHOCK TRANSCRIPTION FACTOR A1 (HSFA1). Acquired thermotolerance was, however, normal in the sumo1/2 knockdown mutant. Thus, SUMO-dependent thermo-resilience is potentially controlled in a different way from the protein damage pathway that underpins thermotolerance. Close inspection of shoot apices revealed that the cell patterning and tissue integrity of the shoot apex of the SUMO1/2 knockdown mutant was lost at 28 °C but not 22 °C. We thus describe a novel SUMO-dependent phenotype. |
| Databáze: | OpenAIRE |
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