| Autor: |
Ruibal, P, Franken, KLMC, van Meijgaarden, KE, Walters, LC, McMichael, AJ, Gillespie, GM, Joosten, SA, Ottenhoff, THM |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Methods in Molecular Biology ISBN: 9781071627112 |
| ISSN: |
1940-6029 |
| Popis: |
Understanding the interactions involved during the immunological synapse between peptide, HLA-E molecules, and TCR is crucial to effectively target protective HLA-E-restricted T-cell responses in humans. Here we describe three techniques based on the generation of MHC-E/peptide complexes (MHC-E generically includes HLA-E-like molecules in human and nonhuman species, while HLA-E specifically refers to human molecules), which allow to investigate MHC-E/peptide binding at the molecular level through binding assays and by using peptide loaded HLA-E tetramers, to detect, isolate, and study peptide-specific HLA-E-restricted human T-cells. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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