Monitoring of RBC rheology after cryopreservation to detect autologous blood doping in vivo? A pilot study

Autor:	Andreas Grolle, Javier Antonio Noriega Urena, Daniel A. Bizjak, Wilhelm Bloch, Marijke Grau, Robert Deitenbeck
Rok vydání:	2020
Předmět:	Adult Male 0301 basic medicine Physiology Autologous blood Pilot Projects Blood volume 030204 cardiovascular system & hematology Cryopreservation Nitric oxide Andrology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo Physiology (medical) medicine Humans Doping in Sports Blood Specimen Collection business.industry Hematology Healthy Volunteers Red blood cell 030104 developmental biology medicine.anatomical_structure chemistry Erythropoietin Hemorheology Rheology Cardiology and Cardiovascular Medicine business medicine.drug
Zdroj:	Clinical Hemorheology and Microcirculation. 76:367-379
ISSN:	1875-8622 1386-0291
Popis:	BACKGROUND: Autologous blood doping (ABD) is applied to improve performance capacity. ABD includes blood donation, red blood cell (RBC) storage at –80°C and re-infusion prior to or during competition. ABD is not directly detectable with current detection techniques. OBJECTIVE: Since cryopreservation is known to affect RBC physiology in vitro, the aim of the study was to examine whether these alterations are detectable in vivo. METHODS: Blood from six healthy male donors was transferred into conventional blood bags, cryopreserved, stored for 18 weeks at –80°C and re-infused with a RBC volume corresponding to ∼4% of total blood volume into respective donor. RBC physiology parameters were measured before blood donation/re-infusion, and 0/1/2/6/24/48/72 h and 1 w post re-infusion. RESULTS: RBC parameters and age markers were unaffected during intervention. RBC deformability increased from pre-blood-sampling to pre-re-infusion while deformability and viscosity values remained unaltered post re-infusion. RBC nitric oxide associated analytes, metabolic parameters and electrolyte concentrations remained unaffected. CONCLUSIONS: The data of this pilot study indicate that the increase in RBC deformability might be related to neoformation of RBC after blood donation. The lack of changes in tested parameters might be related to the low re-infused RBC volume which might explain differences to in vitro results.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::57ed2fce3686bfabbe3955cf0b3f1ee6 https://doi.org/10.3233/ch-200887 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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