Targeted photodynamic therapy using reconstituted high-density lipoproteins as rhodamine transporters
| Autor: | Eugenio Torres-García, Marcela A. Ávila-Sánchez, Keila Isaac-Olivé, Nallely Jiménez-Mancilla, Blanca Ocampo-García, Adriana Plata-Becerril, José Antonio Estrada, Liliana Aranda-Lara, Miguel A. Camacho-López, Clara Santos-Cuevas, Enrique Morales-Avila |
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| Rok vydání: | 2022 |
| Předmět: |
medicine.medical_treatment
media_common.quotation_subject Biophysics Photodynamic therapy Dermatology Targeted therapy Rhodamine chemistry.chemical_compound Cell Line Tumor medicine Humans Cytotoxic T cell Pharmacology (medical) Scavenger receptor Cytotoxicity Internalization media_common Photosensitizing Agents Rhodamines Chemistry In vitro Photochemotherapy Oncology Nanoparticles Lipoproteins HDL |
| Zdroj: | Photodiagnosis and Photodynamic Therapy. 37:102630 |
| ISSN: | 1572-1000 |
| Popis: | Reconstituted high-density lipoprotein (rHDL) nanoparticles are excellent transporters of molecules and very useful for targeted therapy as they specifically recognize the scavenger receptor, class B1 (SR-B1) that is present on the surface of a wide range of tumor cells. However, they have rarely been employed to transport photosensitizers (PS) for photodynamic therapy (PDT). Rhodamine (R) compounds have been dismissed as useful PSs for PDT due to their low 1O2 production, excitation wavelengths with little tissue penetration, and poor selectivity for tumor cells. It was recently demonstrated that when irradiating at 532 nm or with Cerenkov radiation (CR) from a β-emitting radionuclide, R123, R6G, and RB undergo electron transfer reactions (type I reaction) with folic acid. R6G also produces type I reactions with O2. In this work, the photodynamic effects of the rHDL-R system were evaluated in vitro. rHDL nanoparticles loaded with R123, R6G, and RB were synthesized, and the PS was internalized into T47D tumor cells. When cells were irradiated with a 532-nm laser in the presence of an rHDL-R systems, a cytotoxic photodynamic effect was obtained in the order R6G > R123 > RB. In the presence of CR from a 177Lu source, cytotoxicity showed the order R6G > RB > R123. The higher cytotoxicity induced by R6G in both cases corresponds to higher cellular internalization and larger production of type I and II reactions. Thus, in this work, it is proposed that rHDL-R/177Lu system can be applied in theragnostics as a multimodal radiotherapy-PDT-imaging system (imaging by SPECT or Cerenkov) and in hypoxic solid tumors in which external radiation is not effective and 177Lu-CR acts as light source. |
| Databáze: | OpenAIRE |
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