Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda
| Autor: | Dennis R. Burton, Sheila A. Iverson, Stephen J. Benkovic, Michelle A. Alting-Mees, Richard A. Lerner, William D. Huse, Lakshmi Sastry, Angray S. Kang |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
medicine.drug_class
Genetic Vectors Molecular Sequence Data Computational biology Monoclonal antibody Bacteriophage Antigen-Antibody Reactions Immunoglobulin Fab Fragments Mice Organophosphorus Compounds Antibody Repertoire Antibody Specificity medicine Escherichia coli Animals Genomic library Amino Acid Sequence Cloning Molecular Immunoglobulin Fragments Gene Library Multidisciplinary biology Base Sequence Repertoire Gene Amplification Antibodies Monoclonal Lambda phage biology.organism_classification Molecular biology Bacteriophage lambda Recombinant Proteins Hemocyanins Hybridoma technology Hapten |
| Zdroj: | Science (New York, N.Y.). 246(4935) |
| ISSN: | 0036-8075 |
| Popis: | A novel bacteriophage lambda vector system was used to express in Escherichia coli a combinatorial library of Fab fragments of the mouse antibody repertoire. The system allows rapid and easy identification of monoclonal Fab fragments in a form suitable for genetic manipulation. It was possible to generate, in 2 weeks, large numbers of monoclonal Fab fragments against a transition state analog hapten. The methods described may supersede present-day hybridoma technology and facilitate the production of catalytic and other antibodies. |
| Databáze: | OpenAIRE |
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