| Autor: |
Martinez-Valbuena, Ivan, Visanji, Naomi P., Kim, Ain, Lau, Heather H. C., So, Raphaella W. L., Alshimemeri, Sohaila, Gao, Andrew, Seidman, Michael A., Luquin, Maria R., Watts, Joel C., Lang, Anthony E., Kovacs, Gabor G. |
| Rok vydání: |
2022 |
| Předmět: |
|
| DOI: |
10.6084/m9.figshare.19129118 |
| Popis: |
Additional file 1. Fig. S1. The differential interaction of ThT with ��-synuclein aggregates derived from patients with PD or MSA depends on the RT-QuIC reaction buffer. Fig. S2. The levels of total and aggregated ��-synuclein in the Substantia Nigra are relatively uniform between the MSA and the LBD patients included in the validation phase. Fig. S3. End-point dilution alpha-synuclein RT-QuIC analysis of the cerebellar PBS-soluble fraction from two MSA cases classified as high and low seeders. Fig. S4. Dissection of brain regions for protein extraction and ��-synuclein seeding evaluation. Fig. S5. The inter-individual ��-synuclein seeding and intra-individual ��-synuclein seeding behavior is distinct between MSA patients and brain regions. Fig. S6. The levels of total ��-synuclein in each brain region are relatively uniform but the burden of aggregated ��-synuclein varies across patients and brain regions in MSA patients. Fig. S7. The inter-individual but not the intra-individual ��-synuclein seeding heterogeneity is preserved using the sarkosyl insoluble fraction. Fig. S8. The SI fraction promotes a faster aggregation and reaches a higher fluorescence plateau than the PBS-soluble fraction. Fig. S9. The extent of pathology detected by different ��-synuclein antibodies is not uniform. Fig. S10. GCIs and NCIs deposition across different brain regions in MSA patients. Fig. S11. The burden of GCIs and NCIs varies across different brain regions in MSA. Table S1. Demographic and neuropathological diagnosis of the subjects included in this study. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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