Deep viral blood metagenomics reveals extensive anellovirus diversity in healthy humans
| Autor: | Carlos Pena, Luis Larrea, Wladimiro Díaz, Vicente Arnau, Juan Vicente Bou, Cristina Arbona, Rafael Sanjuán, José M. Cuevas, María Cebriá-Mendoza |
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| Přispěvatelé: | Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission, Ministerio de Economía y Competitividad (España), Ministerio de Economía, Industria y Competitividad (España) |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Evolution Science 030106 microbiology Computational biology Biology Anelloviridae Microbiology Article 03 medical and health sciences symbols.namesake Viral sequence Virology Genetics Humans Human virome Taxonomy Sanger sequencing Multidisciplinary Massive parallel sequencing Phylogenetic tree Virome RNA DNA extraction Healthy Volunteers 030104 developmental biology Blood Metagenomics symbols Medicine Microbial genetics |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 2045-2322 |
| Popis: | Human blood metagenomics has revealed the presence of different types of viruses in apparently healthy subjects. By far, anelloviruses constitute the viral family that is more frequently found in human blood, although amplification biases and contaminations pose a major challenge in this field. To investigate this further, we subjected pooled plasma samples from 120 healthy donors in Spain to high-speed centrifugation, RNA and DNA extraction, random amplification, and massive parallel sequencing. Our results confirm the extensive presence of anelloviruses in such samples, which represented nearly 97% of the total viral sequence reads obtained. We assembled 114 different viral genomes belonging to this family, revealing remarkable diversity. Phylogenetic analysis of ORF1 suggested 28 potentially novel anellovirus species, 24 of which were validated by Sanger sequencing to discard artifacts. These findings underscore the importance of implementing more efficient purification procedures that enrich the viral fraction as an essential step in virome studies and question the suggested pathological role of anelloviruses. This work was supported by Grant SAF2017-82287-R from the Spanish Ministry of Economy, Industry and competitiveness (MINECO) and FEDER funding. JMC was supported by a Ramón y Cajal postdoctoral contract from MINECO. |
| Databáze: | OpenAIRE |
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