Beta-Asarone Alleviates Myocardial Ischemia–Reperfusion Injury by Inhibiting Inflammatory Response and NLRP3 Inflammasome Mediated Pyroptosis
| Autor: | Yanchuan Wu, Bin Xiao, Xiaobo Huang, Qian Wang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Inflammasomes Pharmaceutical Science Infarction Allylbenzene Derivatives Myocardial Reperfusion Injury Inflammation Anisoles Pharmacology Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Fibrinolytic Agents NLR Family Pyrin Domain-Containing 3 Protein Pyroptosis medicine Animals Myocardial infarction Ejection fraction biology business.industry Inflammasome General Medicine medicine.disease Rats 030104 developmental biology 030220 oncology & carcinogenesis Myeloperoxidase biology.protein Inflammation Mediators medicine.symptom business Reperfusion injury medicine.drug |
| Zdroj: | Biological and Pharmaceutical Bulletin. 43:1046-1051 |
| ISSN: | 1347-5215 0918-6158 |
| Popis: | Beta-asarone (β-Asarone), the major component of Acorus tatarinowii Rhizoma, has been proved to be muti-pharmacological activities including anti-inflammation, and which is effective in protecting the central nervous system. However, the effect of β-Asarone on myocardial ischemia-reperfusion (I/R) injury is not yet clear. This study used a rat model with 45 min occlusion and 24 h releasing of proximal segment of left anterior descending coronary artery. The effects of β-Asarone on cardiac histopathology, myocardial infarction size, levels of cardiac troponin T (cTNT), myeloperoxidase (MPO) and interleukin-1β (IL-1β), protein expressions of apoptosis-associated speck-like protein containing a CARD (ASC), Nod-like receptor protein 3 (NLRP3), caspase-1 and Gasdermin D (GSDMSD), and left ventricular performance were studied respectively. Our results showed that administration of β-Asarone significantly improved the heart outcome after myocardial ischemia and reperfusion in terms of less infarction size and lower serum cTNT concentration. Further, β-Asarone treatment evidently inhibited inflammatory response with less granulocyte infiltration, mild tissue edema and lower tissue MPO content, it also suppressed NLRP3 signal pathway and cardiac cell's pyroptosis for less protein expressions of ASC and NLRP3, lower level cleavage activation of caspase-1 and GSDMSD, and lower serum IL-1β concentration. Finally, β-Asarone treatment well preserved the left ventricular performance with higher ejection fraction and fractional shortening. The experimental results suggested that β-Asarone was protective against myocardial ischemia-reperfusion injury, in which inhibition of inflammatory response and suppression of NLRP3 inflammasome mediated pyroptosis were supposed to play a vital role. |
| Databáze: | OpenAIRE |
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