RecA homology search is promoted by mechanical stress along the scanned duplex DNA
| Autor: | Mara Prentiss, Yuen-Ling Chan, Claudia Danilowicz, Vincent William Coljee, Julea Vlassakis, Douglas K. Bishop, Alyson Conover, Efraim Feinstein |
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| Rok vydání: | 2011 |
| Předmět: |
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Base pair DNA Single-Stranded Biology 010402 general chemistry 01 natural sciences Homology (biology) 03 medical and health sciences chemistry.chemical_compound Heavy strand D-loop Sequence Homology Nucleic Acid Genetics 030304 developmental biology 0303 health sciences Nucleic Acid Enzymes DNA Molecular biology 0104 chemical sciences Rec A Recombinases Sense strand chemistry Coding strand Biophysics Stress Mechanical |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/gkr855 |
| Popis: | A RecA–single-stranded DNA (RecA–ssDNA) filament searches a genome for sequence homology by rapidly binding and unbinding double-stranded DNA (dsDNA) until homology is found. We demonstrate that pulling on the opposite termini (3′ and 5′) of one of the two DNA strands in a dsDNA molecule stabilizes the normally unstable binding of that dsDNA to non-homologous RecA–ssDNA filaments, whereas pulling on the two 3′, the two 5′, or all four termini does not. We propose that the ‘outgoing’ strand in the dsDNA is extended by strong DNA–protein contacts, whereas the ‘complementary’ strand is extended by the tension on the base pairs that connect the ‘complementary’ strand to the ‘outgoing’ strand. The stress resulting from different levels of tension on its constitutive strands causes rapid dsDNA unbinding unless sufficient homology is present. |
| Databáze: | OpenAIRE |
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