Estimating the energetic contribution of hydrogen bonding to the stability of Candida methylica formate dehydrogenase by using double mutant cycle
| Autor: | J. John Holbrook, Richard B. Sessions, Anthony R. Clarke, Kathleen M. Moreton, Nevin Gill Karagüler |
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| Rok vydání: | 2004 |
| Předmět: |
Threonine
Hydrogen Stereochemistry chemistry.chemical_element Bioengineering Formate dehydrogenase Applied Microbiology and Biotechnology chemistry.chemical_compound Pseudomonas Molecule Homology modeling Guanidine Candida chemistry.chemical_classification Hydrogen bond Wild type Hydrogen Bonding General Medicine Models Theoretical Formate Dehydrogenases Kinetics Enzyme chemistry Biochemistry Mutation Mutagenesis Site-Directed Thermodynamics Software Biotechnology |
| Zdroj: | Biotechnology Letters. 26:1137-1140 |
| ISSN: | 0141-5492 |
| DOI: | 10.1023/b:bile.0000035485.43826.03 |
| Popis: | An homology model of Candida methylica formate dehydrogenase (cm FDH) was constructed based on the Pseudomonas sp. 101 formate dehydrogenase (ps FDH) structure. In wild type cm FDH, Thr169 and Thr226 can form hydrogen bonds with each other. We measured the interaction energy between the two threonines independent of other interactions in the proteins by using a so-called double mutant cycle and assessing the protein stability from the concentration of guanidine hydrochloride needed to denature 50% of the molecules. We conclude that the hydrogen bonds stabilize the wild type protein by -4 kcal mol(-1). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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