One-step White Blood Cell Extracellular Staining Method for Flow Cytometry
| Autor: | Jean-Marc Busnel, Inès Ait Belkacem, Fabrice Malergue, Franck Galland, Pénélope Bourgoin |
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| Přispěvatelé: | Centre d'Immunologie de Marseille - Luminy (CIML), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Beckman Coulter Immunotech, Marseille, France, Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), DUMENIL, Anita |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Lysis
Strategy and Management [SDV]Life Sciences [q-bio] Industrial and Manufacturing Engineering Flow cytometry Sepsis 03 medical and health sciences 0302 clinical medicine White blood cell Lysis buffer medicine Methods Article Leukocytes ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences Venipuncture medicine.diagnostic_test Mechanical Engineering Metals and Alloys medicine.disease 3. Good health Staining [SDV] Life Sciences [q-bio] Red blood cell medicine.anatomical_structure Blood 030220 oncology & carcinogenesis Point-of-care One-step method Biomedical engineering |
| Zdroj: | Bio-protocol Bio-protocol, Bio-protocol LCC, 2021, 11 (16), ⟨10.21769/BioProtoc.4135⟩ Bio-protocol, 2021, 11 (16), ⟨10.21769/BioProtoc.4135⟩ Bio Protoc |
| ISSN: | 2331-8325 |
| Popis: | Flow cytometry is a powerful analytical technique that is increasingly used in scientific investigations and healthcare; however, it requires time-consuming, multi-step sample procedures, which limits its use to specialized laboratories. In this study, we propose a new universal one-step method in which white blood cell staining and red blood cell lysis are carried out in a single step, using a gentle lysis solution mixed with fluorescent antibody conjugates or probes in a dry or liquid format. The blood sample may be obtained from a routine venipuncture or directly from a fingerprick, allowing for near-patient analysis. This procedure enables the analysis of common white blood cell markers as well as markers related to infections or sepsis. This simpler and faster protocol may help to democratize the use of flow cytometry in the research and medical fields. Graphic abstract: [Image: see text] One-step White Blood Cell Extracellular Staining Method for Flow Cytometry. |
| Databáze: | OpenAIRE |
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