Gene expression, protein profiling, and chemotactic activity of infrapatellar fat pad mesenchymal stem cells in pathologies of the knee joint
| Autor: | Maria Concepcion Guisasola, Irene Tirado, Francisco Forriol, Arancha R. Gortazar, Beatriz Bravo, Javier Vaquero |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Male 0301 basic medicine Pathology medicine.medical_specialty Knee Joint Physiology Anterior cruciate ligament Clinical Biochemistry Adipose tissue Young Adult 03 medical and health sciences 0302 clinical medicine Cell Movement Osteoarthritis Humans Medicine Anterior Cruciate Ligament Cells Cultured TIMP1 biology Infrapatellar fat pad business.industry Anterior Cruciate Ligament Injuries Gene Expression Profiling Mesenchymal stem cell Mesenchymal Stem Cells Patella Cell Biology Middle Aged 030104 developmental biology medicine.anatomical_structure Real-time polymerase chain reaction Adipose Tissue RANKL Culture Media Conditioned 030220 oncology & carcinogenesis biology.protein Cytokines Female Tumor necrosis factor alpha Transcriptome business |
| Zdroj: | Journal of Cellular Physiology. 234:18917-18927 |
| ISSN: | 1097-4652 0021-9541 |
| Popis: | The infrapatellar fat pad (IPFP) is a periarticular adipose knee tissue. This tissue contains a large number of mesenchymal stem cells (MSCs). In the present work, we wanted to study the IPFP MSCs and their relationship and differences in two groups, anterior cruciate ligament (ACL) ruptures knees and ostheoarthrosis (OA). The IPFP of 42 patients with OA or ACL rupture were analyzed. Isolation, primary culture, and a genetic and proteomic study of MSCs from IPFP were performed. Gene expression of IL-6, tumor necrosis factor (TNF), IL-8, HSPA1A (Hsp70), CXCL10, RANTES, MMP1, MMP3, TIMP1, and BMP7 was analyzed by real-time quantitative polymerase chain reaction (RT-qPCR). We analyzed MSCs from from 12 diferents patients in two cellular pools (6 from AO disease and 6 from ALC rupture to form two cell pool), for the iTRAQ Proteomic Assay. The conditional media were used in quantitative analysis of MSC soluble factors by Luminex and for de migration assay. A higher gene expression of IL-6, TNF, CXCL10, RANTES, and MMP1 and OPG in MSCs from OA versus ACL (p |
| Databáze: | OpenAIRE |
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