In vivo and in vitro immunosuppressive effects of benzo[k]fluoranthene in female Balb/c mice
| Autor: | In Hye Jun, Ok Hee Kim, Byung Mu Lee, Jun Kyou Kim, Dong Wook Lee, Young Na Yum, Ghee Hwan Kim, Sun Hee Hyun, Tae Won Jeon, Tae Cheon Jeong, Chun Hua Jin, Sangkyu Lee |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
CD4-Positive T-Lymphocytes
Erythrocytes Health Toxicology and Mutagenesis Spleen Thymus Gland CD8-Positive T-Lymphocytes Toxicology BALB/c Mice Immune system In vivo T-Lymphocyte Subsets medicine Splenocyte Animals Antibody-Producing Cells Carcinogen Fluorenes Mice Inbred BALB C Sheep biology biology.organism_classification Flow Cytometry Molecular biology Carcinogens Environmental medicine.anatomical_structure Immunology Interleukin-2 Female CD8 Ex vivo Immunosuppressive Agents |
| Zdroj: | Journal of toxicology and environmental health. Part A. 68(23-24) |
| ISSN: | 1528-7394 |
| Popis: | Although polycyclic aromatic hydrocarbons (PAHs) have been known to suppress immune responses, few studies have addressed the immunotoxicity of benzo[k]fluoranthene (B[k]F). In this study, we investigated the immunosuppression by B[k]F, both in vivo and in vitro, in female BALB/c mice. To assess the effects of B[k]F on humoral immunity as splenic antibody response to sheep red blood cells (SRBCs), B[k]F was given a single dose or once daily for 7 consecutive days po with 30, 60, and 120 micromol/kg. B[k]F reduced the number of antibody-forming cells (AFCs) in a dose-dependent manner. Subacute treatment with B[k]F caused weight increases in liver and decreases in spleen and thymus. The number of AFCs was dramatically decreased by B[k]F in a dose-dependent manner. In a subsequent study, mice were subacutely exposed to the same doses of B[k]F without an immunization with SRBCs, followed by splenic and thymic lymphocyte phenotypings using a flow cytometry and ex vivo mitogen-stimulated proliferation. B[k]F-exposed mice exhibited reduced splenic and thymic cellularity, decreased numbers of total T cells, CD4(+) cells, and CD8(+) cells in spleen, and immature CD4(+)CD8(+) cells, CD4(+)CD8(-) cells, and CD8(+)CD4(-) cells in thymus. The number of CD4(+) IL-2(+) cells was reduced by about 11%, 31%, and 53% following exposure of mice to 30, 60, and 120 micromol/kg of B[k]F, respectively. In the ex vivo lymphocyte proliferation assay, B[k]F inhibited splenocyte proliferation by LPS and Con A. In the in vitro mitogen-stimulated proliferation by untreated splenic suspensions, B[k]F only suppressed splenocyte proliferation to LPS. These results suggested that B[k]F-induced immunosuppression might be mediated, at least in part, through the IL-2 production, and caused by mechanisms associated with metabolic processes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|