Nutrient load acts as a driver of gut microbiota load, community composition and metabolic functionality in the simulator of the human intestinal microbial ecosystem
| Autor: | Jeroen Raes, Kim De Paepe, Yorick Minnebo, Tom Van de Wiele |
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| Rok vydání: | 2021 |
| Předmět: |
BACTERIAL
MUCIN Metabolite PATHOGENESIS DIVERSITY gut microbiome Biology Gut flora BILOPHILA-WADSWORTHIA Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound Nutrient Verrucomicrobia microbial quantity Humans REACTOR Food science Microbiome SHIME in vitro gut simulator nutrient concentration chemistry.chemical_classification Science & Technology Ecology Microbiota Fatty acid IN-VITRO Nutrients Fatty Acids Volatile biology.organism_classification Gastrointestinal Microbiome gut microbial ecology Bilophila wadsworthia CHAIN FATTY-ACIDS Microbial population biology chemistry PATTERNS GROWTH Composition (visual arts) quantitative microbiome profiling Life Sciences & Biomedicine |
| Zdroj: | FEMS Microbiology Ecology. 97 |
| ISSN: | 1574-6941 |
| Popis: | A recently introduced quantitative framework for gut microbiota analysis indicated that microbial load alterations can be linked to various diseases, making it essential to pinpoint its determinants. We identified nutrient load as a main driver of the quantitative microbial community composition and functionality in vitro by stepwise decreasing standardized feed concentrations from 100% to 33%, 20% and 10% in 5-day intervals. While the proportional composition and metabolic profile were mainly determined by the inter-individual variability (35% and 41%), nutrient load accounted for 58%, 23% and 65% of the observed variation in the microbial load, quantitative composition and net daily metabolite production, respectively. After the 10-fold nutrient reduction, the microbial load decreased by 79.72 ± 9% and 82.96 ± 1.66% in the proximal and distal colon, respectively, while the net total short-chain fatty acid production dropped by 79.42 ± 4.42% and 84.58 ± 2.42%, respectively. The majority of microbial taxa quantitatively decreased, whereas a select group of nutritional specialists, such as Akkermansia muciniphila and Bilophila wadsworthia, and a number of opportunistic pathogens remained unaffected. This shows that nutrient load is an important driver of the human gut microbiome and should be considered in future in vitro and in vivo dietary research. ispartof: FEMS MICROBIOLOGY ECOLOGY vol:97 issue:9 ispartof: location:England status: published |
| Databáze: | OpenAIRE |
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