Possible genetic damage in the Czech nuclear power plant workers
| Autor: | Pavel Rossner, Hana Bavorová, Dana Očadlíková, Alena Milcova, Petra Musilova, Jana Schmuczerova, Radim J. Sram, Irena Chvatalova, Jiri Rubes, Olga Kopecna, Olena Beskid, Zdik Dusek, Ivo Solansky |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Health Toxicology and Mutagenesis Chromosomal translocation Ionizing radiation Dicentric chromosome Occupational Exposure Genetics medicine Humans Methionine synthase Cotinine Molecular Biology In Situ Hybridization Fluorescence Czech Republic Chromosome Aberrations biology medicine.diagnostic_test Chromosome Molecular biology Case-Control Studies Methylenetetrahydrofolate reductase biology.protein Chromatid Power Plants Fluorescence in situ hybridization |
| Zdroj: | Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 593:50-63 |
| ISSN: | 0027-5107 |
| Popis: | The aim of our study was to identify occupational risk of irradiation exposure in the Czech nuclear power plant workers. We analyzed levels of chromosomal aberrations, a well-known biomarker of early biological effects and a predictor of cancer risk. We applied the conventional method of cytogenetic analysis and fluorescence in situ hybridization (FISH, whole chromosome painting for chromosomes 1 and 4, combined with a pancentromeric probe) to three groups: 123 subjects in the Temelin nuclear power plant (2 years in use), 114 subjects in the Dukovany nuclear power plant (20 years in use), and 53 matched controls from Ceske Budejovice. Nuclear power plant workers were divided into two groups: subjects with admittance into the monitored zone, and others. Following factors were also analyzed: GSTM1, GSTT1, GSTP1, XPD, XRCC1, hOGG1, p53, MTHFR, and MS gene polymorphisms, levels of vitamins A, C, E, and folate in plasma, and level of cotinine in urine. Long-term exposure to ionizing radiation in the monitored zone was 0.47 ± 1.50 mSv (miliSievert) in the Temelin nuclear power plant and 5.74 ± 9.57 mSv in the Dukovany nuclear power plant. Using the conventional cytogenetic analysis, we observed 1.90 ± 0.95 and 1.82 ± 1.19% AB.C. (percent of aberrant cells) in the Temelin nuclear power plant, and 2.39 ± 1.01 and 2.33 ± 1.04% AB.C. in the Dukovany nuclear power plant, for monitored zone workers and others, respectively. In the control group, we found 2.25 ± 0.82% AB.C. Genomic frequency of translocations FG/100 measured by FISH was 1.89± 1.40 and 2.01 ± 1.68 in the Temelin nuclear power Abbreviations: AB.C., aberrant cells; FISH, fluorescence in situ hybridization; IR, ionizing radiation; mSv, miliSievert, unit of the radiation equivalent dose; NPP, nuclear power plant; PAINT, protocol for aberrations identification and nomenclature; PBL, peripheral blood lymphocytes; NS, nonsmokers; SM, smokers; GST, glutathione-S-transferase; hOGG1, 8-oxoguanine DNA glycosylase; MTHFR, methylene tetrahydrofolate reductase; MS, methionine synthase; B� , chromatid break; B�� , chromosome break; B/C, breaks/cell; Ex� , chromatid exchange; Ex�� , chromosome exchange; ace, acentric chromosome; dic, dicentric chromosome; ins, insertions; NCJ, number of color junctions; FG/100, genomic frequency of translocations in 100 cells; rcp, reciprocal translocations; t, translocations |
| Databáze: | OpenAIRE |
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