Molecular cloning of the cDNA encoding a stress-inducible protein phosphatase 1 (PP1) catalytic subunit from French bean (Phaseolus vulgaris L.)
| Autor: | S Jupe, Bolwell Gp, Alfred Zimmerlin |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
DNA
Complementary Protein subunit Molecular Sequence Data Phosphatase Plant Science Molecular cloning Biology Catalysis Protein Phosphatase 1 Complementary DNA Phosphoprotein Phosphatases Genetics Amino Acid Sequence RNA Messenger Cloning Molecular Plant Proteins Plants Medicinal Sequence Homology Amino Acid cDNA library food and beverages Fabaceae Protein phosphatase 1 General Medicine Blotting Northern Molecular biology Open reading frame Plant protein Enzyme Induction Agronomy and Crop Science |
| Zdroj: | Plant Molecular Biology. 28:363-368 |
| ISSN: | 1573-5028 0167-4412 |
| DOI: | 10.1007/bf00020386 |
| Popis: | A cDNA showing high sequence similarity (>70%) to plant protein phosphatase 1 catalytic subunit variants from other species has been isolated from a cDNA library derived from mRNAs expressed in elicitor-treated suspension-cultured cells. The clone appears to be a near full-length 1431 bp with a 172 bp 5′-untranslated region and a 317 bp 3′-untranslated region. The open reading frame, determined by sequence similarity, codes for a protein with predicted M r of 35552. Alternatively an ATG situated to the 5′ end of the putative start site would increase the protein size by 6 amino acids. The mRNA for Pvpp1 was shown to be rapidly induced by elicitor treatment of suspension-cultured cells of French bean. The cloned cDNA represents one of the few examples of a gene product that is probably involved in dephosphorylation events arising after the initial responses to biotic stress. |
| Databáze: | OpenAIRE |
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