Magnetic Mesoporous Silica Nanorods Loaded with Ceria and Functionalized with Fluorophores for Multimodal Imaging

Autor: Jan Grzelak, Jaume Gázquez, Alba Grayston, Mariana Teles, Fernando Herranz, Nerea Roher, Anna Rosell, Anna Roig, Martí Gich
Přispěvatelé: Ministerio de Ciencia e Innovación (España), Generalitat de Catalunya, European Cooperation in Science and Technology, Herranz, Fernando, Roig Serra, Anna, Gich, Martí, Herranz, Fernando [0000-0002-3743-0050], Roig Serra, Anna [0000-0001-6464-7573], Gich, Martí [0000-0001-9958-0057]
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
instname
Dipòsit Digital de Documents de la UAB
Universitat Autònoma de Barcelona
Popis: Multifunctional magnetic nanocomposites based on mesoporous silica have a wide range of potential applications in catalysis, biomedicine, or sensing. Such particles combine responsiveness to external magnetic fields with other functionalities endowed by the agents loaded inside the pores or conjugated to the particle surface. Different applications might benefit from specific particle morphologies. In the case of biomedical applications, mesoporous silica nanospheres have been extensively studied while nanorods, with a more challenging preparation, have attracted much less attention despite the positive impact on the therapeutic performance shown by seminal studies. Here, we report on a sol-gel synthesis of mesoporous rodlike silica particles of two distinct lengths (1.4 and 0.9 μm) and aspect ratios (4.7 and 2.2) using Pluronic P123 as a structure-directing template and rendering ∼1 g of rods per batch. Iron oxide nanoparticles have been synthesized within the pores yielding maghemite (γ-Fe2O3) nanocrystals of elongated shape (∼7 nm × 5 nm) with a [110] preferential orientation along the rod axis and a superparamagnetic character. The performance of the rods as T2-weighted MRI contrast agents has also been confirmed. In a subsequent step, the mesoporous silica rods were loaded with a cerium compound and their surface was functionalized with fluorophores (fluorescamine and Cyanine5) emitting at λ = 525 and 730 nm, respectively, thus highlighting the possibility of multiple imaging modalities. The biocompatibility of the rods was evaluated in vitro in a zebrafish (Danio rerio) liver cell line (ZFL), with results showing that neither long nor short rods with magnetic particles caused cytotoxicity in ZFL cells for concentrations up to 50 μg/ml. We advocate that such nanocomposites can find applications in medical imaging and therapy, where the influence of shape on performance can be also assessed.
The authors acknowledge financial support from the Spanish Ministry of Science and Innovation through the RTI2018-096273-B-I00 project, the ‘Severo Ochoa’ Programme for Centers of Excellence in R&D (CEX2019-000917-S). The Generalitat de Catalunya, project 2017SGR765, is also acknowledged. This research work was performed in the framework of the Nanomedicine CSIC HUB (ref 202180E048).The authors participate in the Aerogels COST ACTION (CA 18125). J.G. received financial support through the “la Caixa” INPhINIT Fellowship Grant for Doctoral Studies at Spanish Research Centers of Excellence (Grant code: LCF/BQ/DI17/11620041), “la Caixa” Banking Foundation (ID100010434), Barcelona, Spain. J.G. was enrolled in the doctoral program in Materials Science at the UAB. M.T. (ref RYC2019-026841-I) has a post-doctoral fellowship “Ramón y Cajal” supported by the “Ministerio de Ciencia e Innovación”, Spanish Government. A. G. has been supported by the fellowship from Instituto de Salud Carlos III with FEDER funds (FI17/00073). A.Rosell and A. G. work takes part within the RETICS-INVICTUS PLUS from Instituto de Salud Carlos III with FEDER funds (RD16/0019/0021). G. Antorrena from the LMA at Universidad de Zaragoza is acknowledged for performing XPS measurements. ICTS-CNME at UCM is also acknowledged for offering access to STEM and expertise. The ICMAB Scientific services of electron microscopy, low temperatures and magnetometry, thermal analysis, and X-ray diffraction have performed measurements for the present work. Nico Dix, Joan Esquius, and Xavi Campos are acknowledged by making possible SAXS measurements at the ICMAB X-ray diffraction service.
Databáze: OpenAIRE
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