Unique Functional Properties of Mature Adult-Born Neurons in the Mouse Olfactory Bulb
| Autor: | Nima Mojtahedi, Stefan Fink, Elizabeta Zirdum, Astrid Alsema, Olga Garaschuk, Natalie Fomin-Thunemann, Yury Kovalchuk |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cell Methysergide Biology Biochemistry Article Mice odor responses 03 medical and health sciences 0302 clinical medicine Interneurons GABAergic cells adult-born cells Genetics medicine Animals 5-hydroxytryptamin GABAergic Neurons 5-HT receptor in vivo calcium imaging Antagonist Cell Biology Olfactory Bulb acetylcholine serotonin Olfactory bulb Cell biology 030104 developmental biology medicine.anatomical_structure Odorants noradrenaline GABAergic awake mice Serotonin 030217 neurology & neurosurgery Acetylcholine Developmental Biology medicine.drug |
| Zdroj: | Stem Cell Reports |
| ISSN: | 2213-6711 |
| Popis: | Summary The rodent olfactory bulb (OB) is continuously supplied with adult-born cells maturing into GABAergic neurons. Using in vivo ratiometric Ca2+ imaging to readout ongoing and sensory-driven activity, we asked whether mature adult-born cells (mABCs) in the glomerular layer of the bulb become functionally identical to resident GABAergic (ResGABA) neurons. In awake head-restrained mice the two cell populations differed significantly in terms of ongoing spontaneous activity, with 24% of mABCs contributing to a strongly active cell cluster, absent among ResGABA cells. Odor-evoked responses of mABCs were sparse, less reliable, and had smaller amplitudes compared with ResGABA cells. The opposite was seen under anesthesia, with response reliability increasing and response size of mABCs becoming larger than that of ResGABA cells. Furthermore, ongoing activity of mABCs showed increased sensitivity to ketamine/xylazine and was selectively blocked by the antagonist of serotonin receptors methysergide. These functional features of mABCs clearly distinguish them from other OB interneurons. Highlights • In awake mice mature adult-born neurons differ from resident GABAergic cells • 24% of neurons belong to a strongly active cell cluster, absent among resident cells • Serotonergic inputs target adult-born but not resident GABAergic cells In this article, Garaschuk and colleagues use in vivo ratiometric calcium imaging to show that mature adult-born cells in the glomerular layer of the mouse olfactory bulb differ from resident GABAergic neurons in terms of spontaneous ongoing activity, odor-evoked responses, sensitivity to ketamine/xylazine anesthesia, and unique serotonergic inputs. |
| Databáze: | OpenAIRE |
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