MicroRNAs as potential biomarkers in congenital heart surgery
| Autor: | Giovanni Biglino, Kerrie L. Ford, Serban Stoica, Antonella Vardeu, Massimo Caputo, Mustafa Zakkar, Costanza Emanueli, Domenico Bruno, Andrew D Mumford, Gianni D Angelini, Dan M Dorobantu |
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| Rok vydání: | 2020 |
| Předmět: |
Heart Defects
Congenital Male Pulmonary and Respiratory Medicine medicine.medical_specialty pediatric cardiac surgery 030204 cardiovascular system & hematology outcome prediction Intracardiac injection law.invention Cohort Studies 03 medical and health sciences Postoperative Complications 0302 clinical medicine Predictive Value of Tests law Cardiopulmonary bypass Humans Medicine Cardiac Surgical Procedures Pediatric intensive care unit business.industry Infant biomarkers Perioperative Length of Stay Hypoxia (medical) congenital heart disease microRNAs Cardiac surgery Surgery MicroRNAs Real-time polymerase chain reaction 030228 respiratory system Centre for Surgical Research Child Preschool Cohort Female medicine.symptom Cardiology and Cardiovascular Medicine business Biomarkers |
| Zdroj: | Stoica, S C, Dorobantu, D M, Vardeu, A, Biglino, G, Ford, K L, Bruno, D V, Zakkar, M, Mumford, A, Angelini, G D, Caputo, M & Emanueli, C 2019, ' MicroRNAs as potential biomarkers in congenital heart surgery ', Journal of Thoracic and Cardiovascular Surgery . https://doi.org/10.1016/j.jtcvs.2019.03.062 |
| ISSN: | 0022-5223 |
| DOI: | 10.1016/j.jtcvs.2019.03.062 |
| Popis: | Objective: Pediatric congenital heart surgery (CHS)involves intracardiac, valvular, and vascular repairs. Accurate tools to aid short-term outcome prediction in pediatric CHS are lacking. Clinical scores, such as the vasoactive-inotrope score and ventilation index, are used to define outcome in clinical studies. MicroRNA-1-3p (miR-1)is expressed by both cardiomyocytes and vascular cells and is regulated by hypoxia. In adult patients, miR-1 increases in the circulation after open-heart cardiac surgery, suggesting its potential as a clinical biomarker. Thus, we investigated whether perioperative circulating miR-1 measurements can help predict post-CHS short-term outcomes in pediatric patients. Methods: Plasma miR-1 was retrospectively measured in a cohort of 199 consecutive pediatric CHS patients (median age 1.2 years). Samples were taken before surgery and at the end of the operation. Plasma miR-1 concentration was measured by reverse transcription-quantitative polymerase chain reaction and expressed as miR-1 copies/μL and as relative expression to spiked-in exogenous cel-miR-39. Results: Baseline plasma miR-1 did not vary across different diagnoses, increased during surgery (204-fold median relative increase, P Conclusions: Our study suggests miR-1 as a novel potential circulating biomarker to predict early postoperative outcome and inform clinical management in pediatric heart surgery. |
| Databáze: | OpenAIRE |
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